Antibodies against Proteins/Bioactives/Marke|
|species reactivity||human, chicken, rat, mouse, quail, bovine|
|western blot: 1-2 μg/mL using rat brain extract|
|antibody form||purified immunoglobulin|
|form||buffered aqueous solution|
|mol wt||antigen mol wt ~280 kDa|
|shipped in||dry ice|
human ... MAP2(4133)|
mouse ... Mtap2(17756)
rat ... Map2(25595)
rat brain microtubule-associated proteins (MAPs).
MAP2 is the major microtubule associated protein of brain tissue. There are three forms of MAP2; two are similarly sized with apparent molecular weights of 280 kDa (MAP2a and b) and the third with a lower molecular weight of 70 kDa (MAP2c). In the newborn rat brain, MAP2b and MAP2c are present while MAP2a is absent. Between postnatal days 10 and 20, MAP2a appears at levels equal to that of MAP2b. At the same time, the level of MAP2c drops by 10-fold. This change happens during the period when dendrite growth is completed and neurons have reached their mature morphology. MAP2 is known to promote microtubule assembly and to form side arms on microtubules. It also interacts with neurofilaments, actin and other elements of the cytoskeleton.
Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.
Monoclonal Anti-MAP2 antibody recognizes all known forms of MAP2, namely MAP2a, MAP2b and MAP2c. It does not cross-react with other MAPs or tubulin. By immunohistochemical staining of formalin-fixed, paraffin-embedded brain tissue, the antibody shows selective labeling of dendritic trees throughout the brain.
Reacts with all known forms of MAP2, namely MAP2a, MAP2b and MAP2c. Does not cross-react with other MAPs or tubulin. By immunohistochemical staining of formalin-fixed, paraffin-embedded brain tissue, the antibody shows selective labeling of dendritic trees throughout the brain.
Monoclonal Anti-MAP2 antibody may be used in various immunochemical techniques including immunoblotting, immunohistochemistry, and immunocytochemistry. Monoclonals antibodies to MAP2 are used to study the cytoskeleton structure in cells.
By immunoblotting, a working antibody concentration of 1-2 μg/ml is recommended using a rat brain extract.
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Keywords: Buffers, Cell biology, Epigenetics, Immunohistochemistry, Immunoprecipitation, Molecular biology, Neuroscience, Western blot
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