Synonym: Acyl-neuraminyl Hydrolase, Neuraminidase from Arthrobacter ureafaciens, Sialidase
|Related Categories||3.2.x.x Glycosidases, 3.x.x.x Hydrolases, Antibody Characterization and Analysis, Antibody Glycan Analysis, Application Index,|
|recombinant||expressed in E. coli|
|form||buffered aqueous solution|
|activity||≥135 units/mg protein|
|foreign activity||β-Galactosidase, α-mannosidase, β-hexosaminidase, α-fucosidase, and proteases, none detected|
Releases α(2→3), α(2→6), α(2→8), and α(2→9)-linked N-acetylneuraminic acid from complex oligosaccharides.
Provided with 5× reaction buffer (250 mM sodium phosphate, pH 6.0).
Expressed in glycosidase-free hosts.
One unit will hydrolyze 1 μmole of 4-methylumbelliferyl α-
Solution in 20 mM Tris-HCl, pH 7.5, and 20 mM NaCl.
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