|Related Categories||Antibody Arrays, Cell Biology, Neurobiology Array, Panorama Antibody Array- Neurobiology, Protein Arrays More...|
|species reactivity||human, mouse, rat|
|shipped in||wet ice|
224 antibodies spotted in 32 sub-arrays, each containing duplicate spots of the antibodies, as well as duplicate positive control spots (a monoclonal antibody that recognizes Cy3 and Cy5) and several negative controls. Antibodies are spotted in high density to ensure strong signals. Antibodies are also available individually from Sigma for more extensive analysis of your sample. Refer to Antibody List for specific catalog number(s). sigma.com/nbaa5
Cell or tissue extracts, blood plasma or serum
Please note that featured antibody content is lot specific and is subject to change.
For more information see Protein Array Literature References
Nitrocellulose glass slide, proprietary treatment to minimize background staining.
The array can be used for comparing protein expression profiles between two samples (test vs. reference samples). Each sample is labeled with a different dye (such as Cy3 or Cy5) and the 2 samples are applied simultaneously on the array, The expression profile of each sample is recorded individually and compared.
The Panorama Antibody Microarray - Neurobiology is a proteomics tool to measure the relative level of protein expression in cell or tissue extracts. The array contains 224 different antibodies on nitrocellulose coated glass slides. These antibodies represent targets to highly studied neurodegenerative diseases, such as Alzheimer′s, Huntington′s, and Parkinson′s. Also represented on the array are targets involved in neuron development.
Panorama is a registered trademark of Sigma-Aldrich Co. LLC
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|Panorama Antibody Slide- Neurobiology|
|QuadiPERM Cell Culture Vessel 2EA|
|Protease Inhibitor Cocktail 1VL|
|Benzonase®, Ultrapure 1000UN|
|Array Incubation Buffer 20ML|
|SigmaSpin Post-Reaction Clean-Up Columns 8EA|
|Microcentrifuge collection tubes 16EA|
|Panorama Antibody List- Neurobiology 1EA|
Certificate of Analysis
Antibody microarrays are used for profiling complex protein samples and for assessing differential protein expression. The Panorama Ab Microarray kits, consisting of a series of arrays that cover var...
Carolyn L. Crankshaw
BioFiles v7 n2, 2011, 21–22
Keywords: AGE, Antibody microarrays, Cell signaling, Gene expression, Microarray Analysis, PAGE, Western blot
While the two-color antibody microarray is a relatively new technology, the basic data analysis principles are the same as for DNA microarrays. For both types of arrays it is highly desirable to perf...
Keywords: Antibody microarrays, DNA microarrays, Gene expression, Microarray Analysis, Polymerase chain reaction, Western blot
Before performing the labeling procedure (see Sample Labeling and Processing) it is important to bring the extract to the required pH by dialysis.
Keywords: Centrifugation, Dialysis
Detergents can interfere with the labeling efficiency of the extracted proteins. Therefore, a procedure that does not include the use of detergents should be used for the preparation of nuclear prote...
Keywords: Cell disruption, Detergents, Dialysis, Homogenization, Protein extraction
Note: The following procedure is used for extracting proteins from mouse brain and may be suitable for use with other soft tissues.
Before starting the array assay, make sure that the dye to protein molar ratio (D/P) is > 2. A lower ratio may work; however, it may cause background problems.
Keywords: Cell culture
Use freshly prepared extracts for protein labeling is highly recommended. Use of extracts from frozen tissues or cell lines with low viability, or old protein extracts may give inadequate results. Fo...
Keywords: Centrifugation, Dialysis, Extinction coefficient
Each sub-array contains two spots with a monoclonal antibody specific for Cy3/Cy5, which serve as positive controls. These points can be used as internal references for positioning the sub-arrays. Fu...
Identification of human T cell leukemia virus type 1 tax amino acid signals and cellular factors involved in secretion of the viral oncoprotein Jain, P., et al. J. Biol. Chem. 282, 34581-34593, (2007)
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