Polyinosinic–polycytidylic acid sodium salt
DOWNLOAD MSDS (PDF)Synonym: Poly (I:C), Poly(I) • Poly(C)
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CAS Number 42424-50-0
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MDL number MFCD00131984
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Popular Documents: Product Information Sheet (PDF) | Specification Sheet (PDF)
Properties
| Related Categories | Biochemicals and Reagents, Biomaterials, Cell Culture, Materials Science, Nucleosides, Nucleotides, Oligonucleotides, |
| Quality Level | PREMIUM |
| assay | ≥99% (less than 1% free nucleotides, TLC) |
| form | lyophilized powder |
| storage condition | desiccated |
| solubility | H2O: soluble10 mg/mL |
| storage temp. | −20°C |
Description
Biochem/physiol Actions
Transfection of Poly (I:C) into NIT-1 cells has been used as a model of intracellular dsRNA-induced β cell apoptosis. Eighteen hours post transfection, 45% of the cells were apoptotic with an increase in NF-kB, p50/p65 nuclear translocation, and cleavage of caspases 3 and 8, as well as transcriptional induction of caspase 12, Fas, IL-15, and the TNF receptor-associated ligand (TRAIL). It has been suggested that Poly(I:C) is one of the most appropriate generators of stable mature dendritic cells (DC). These mature DC might generate in vivo effective immune responses after injection due to their ability to secrete bioactive IL-12 after CD40 ligation. Poly (I:C) was used as a potent adjuvant to enhance the specific anti-tumor immune responses against a peptide-based vaccine.
Other Notes
Double-stranded homopolymer.
Preparation Note
The product requires ionic strength to maintain the double-strand structure. To prevent denaturation, reconstitute in solutions with physiological salt concentrations (e.g., saline solution). Reconstitution may require heating (50 °C) and cooling to achieve re-annealing.
Application
TLR3 recognizes double-stranded RNA and is a major effector of the immune response against viral pathogens. Polyinosinic–polycytidyli
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