Anti-Protein Kinase Cα antibody produced in rabbit

Immunogen

Synthetic peptide corresponding to amino acids 659-672 from the C-terminal variable (V5) region of rat PKC α conjugated to KLH.

synthetic peptide corresponding to amino acids 659-672 from the C-terminal variable (V5) region of rat PKCα.

General description

PKC α is a conventional isoform of Protein Kinase C (76-93 kD) and a member of serine/threonine (Ser/Thr) protein kinases. It is expressed ubiquitously in tissues and is the major isoform of PKC in fibroblasts. Stimulation and overexpression of PKC α increases the growth rate of cells in culture⁴. Anti-Protein Kinase C α antibody is useful for studying expression of PKC α in normal and neoplastic tissues. It is also useful for detecting PKC α isoenzyme in brain tissue and cell extracts. Furthermore, it can be used in chemiluminescence detection systems to detect PKC α. Rabbit anti-protein kinase c α antibody reacts specifically with PKC α (80 kD) of rat brain extract or NIH3T3 mouse fibroblast lysate.

Protein Kinase C (PKC, 76-93 kD) is a family of Ser/Thr specific protein kinases that perform key functions in numerous signalling pathways, in biological systems, through their various isoforms. The conventional PKC isoforms (cPKC) are PKC-α, β1, β2 and γ; activated by phosphatidylserine, calcium or phorbol esters. Proteolysis of PKC ^{in vivo} is thought to be mediated by calpains I and II. Calpains cleave PKC in the V3 hinge region to produce two distinct fragments, one comprising the N-terminal regulatory domain (30 kD) and the other fragment containing the C-terminal kinase domain (50 kD) that is catalytically active. Multiple functions such as, cellular and vascular regulations, angiogenesis, cell growth, apoptosis, changes in basement membrane thickness, extracellular matrix organisation, MAPK signalling, are attributed to PKC isoforms. These varied functions implicate PKC isoforms in cardiac hypertrophies and diabetic nephropathy and cardiovascular complications.
Anti-Protein Kinase C α antibody specifically recognises PKC α (80 kDa). A faint band at 45 kDa may be observed.

Application

Anti-Protein Kinase C α may be used for detection in rat brain extract and mouse NIH 3T3 fibroblast lysate by immunoblotting at a working dilution of 1:50,000-1:20,000. Detection by immunoblotting is also possible in baby hamster kidney cells (1:10000) ¹ and in common voles ². Detection by immunohistochemistry in retinal sections of European moles is possible at a working dilution of 1:10000 ³. The antibody is also suitable for protein microarray.

Anti-protein kinase c α antibody can be used in immunohistochemical staining and microarray assays. It can also be used in immunoblotting¹ and western blotting.