|Related Categories||1.11.x.x Peroxide as receptor, 1.x.x.x Oxidoreductases, Analytical and Industrial Enzymes, Antibodies, Antibody Labeling,|
|form||essentially salt-free, lyophilized powder|
The RZ (Reinheitszahl) is the absorbance ratio A403/A275 determined at 0.5-1.0 mg/ml in deionized water. It is a measure of hemin content, not enzymatic activity. Even preparations with high RZ may have low enzymatic activity.
This product is assayed using ABTS for easy comparison to other suppliers′ unit: approx. 1,000 units per mg solid
Similar to P8375
Packaged in mg solid
One ABTS unit will oxidize 1 μmole of ABTS per minute at 25 °C at pH 5.0
One pyrogallol unit will form 1.0 mg purpurogallin from pyrogallol in 20 sec at pH 6.0 at 20 °C.
Horseradish peroxidase (HRP) is isolated from horseradish roots (Amoracia rusticana). It is used in biochemistry applications such as western blots, ELISA and Immunohistochemistry. Horseradish peroxidase is used to amplify a weak signal and increase detectability of a target molecule, such as a protein. Product P6782 is type VI-A and premium quailty. It is essentially a salt-free, lyophilized powder. It is used to quantify cholesterol and cholesterol esters1 and to study in vitro lipid deposition on hydrogel and silicone hydrogel contact lenses2.
When incubated with a substrate, horseradish peroxidase produces a coloured, fluorimetric, or luminescent derivative of the labeled molecule, allowing quantification. Horseradish peroxidase has been shown to slightly reduce the level of inhibition in a cydAB mutant.
Horseradish peroxidase is isolated from horseradish roots (Amoracia rusticana) and belongs to the ferroprotoporphyrin group of peroxidases. HRP is a single chain polypeptide containing four disulfide bridges. It is a glycoprotein containing 18% carbohydrate. The carbohydrate composition consists of galactose, arabinose, xylose, fucose, mannose, mannosamine, and galactosamine depending upon the specific isozyme. Its molecular weight (~44 kDa) includes the polypeptide chain (33,890 Daltons), hemin plus Ca2+ (~700 Daltons), and carbohydrate (~9,400 Daltons). At least seven isozymes of HRP exist. The isoelectric point for horseradish peroxidase isozymes ranges from 3.0 - 9.0.
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Type VI, essentially salt-free, lyophilized powder, 250-330 units/mg solid (using pyrogallol)
Type II, essentially salt-free, lyophilized powder, 150-250 units/mg solid (using pyrogallol)
Type I, essentially salt-free, lyophilized powder, 50-150 units/mg solid (using pyrogallol)
Type XII, essentially salt-free, lyophilized powder, 250-330 units/mg solid (using pyrogallol)
Certificate of Analysis
Certificate of Origin
Replaces SPABTS02. Procedure updated to conform to current QUMAS formatting guidelines. Refer to CR SOP-DEK-ENZ42.
Keywords: Extinction coefficient
1. OBJECTIVE To standardize a procedure for the enzymatic determination of Peroxidase by Pyrogallol units.
Keywords: Enzymology, Extinction coefficient, Gene expression, Size-exclusion chromatography
As a leading supplier of antibodies, Sigma® Life Science is committed to providing researchers the antibody they need with the quality they deserve. Our Antibody Explorer Search Tool provides easy ac...
Keywords: Buffers, Cell biology, Epigenetics, Immunohistochemistry, Immunoprecipitation, Molecular biology, Neuroscience, Western blot
Export of functional Streptomyces coelicolor alditol oxidase to the periplasm or cell surface of Escherichia coli and its application in whole-cell biocatalysis. van Bloois E Appl. Microbiol. Biotechnol. 83(4), 679-87, (2009)
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|D9143||o-Dianisidine, peroxidase substrate|
|A4685||Luminol sodium salt|
|A1888||2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt, ≥98% (HPLC)|
|G5502||Guaiacol, oxidation indicator|
|A3537||5-Aminosalicylic acid, ≥99%|
|A4382||4-Aminoantipyrine, reagent grade|
|A8511||Luminol, ≥97% (HPLC)|
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