|Related Categories||1.11.x.x Peroxide as receptor, 1.x.x.x Oxidoreductases, Analytical and Industrial Enzymes, Antibodies, Antibody Labeling,|
|form||essentially salt-free, lyophilized powder|
|solubility||0.1 M phosphate buffer: soluble10 mg/mL, clear, orange to red (pH 6.0)|
The RZ (Reinheitszahl) is the absorbance ratio A403/A275 determined at 0.5-1.0 mg/ml in deionized water. It is a measure of hemin content, not enzymatic activity. Even preparations with high RZ may have low enzymatic activity.
This product is assayed using ABTS for easy comparison to other suppliers′ unit: approx. 1,000 units per mg solid
Similar to P8375
Packaged in mg solid
The product is a 1 mg/mL solution in 0.1 M phosphate buffer(pH 6.0) and remains active for at least two weeks at room temperature. The solution retains activity after 5 freeze-thaw cycles.
One ABTS unit will oxidize 1 μmole of ABTS per minute at 25 °C at pH 5.0
One pyrogallol unit will form 1.0 mg purpurogallin from pyrogallol in 20 sec at pH 6.0 at 20 °C.
Horseradish peroxidase (HRP) is isolated from horseradish roots (Amoracia rusticana). It is used in biochemistry applications such as western blots, ELISA and Immunohistochemistry. Horseradish peroxidase is used to amplify a weak signal and increase detectability of a target molecule, such as a protein. Product P6782 is type VI-A and premium quailty. It is essentially a salt-free, lyophilized powder. It is used to quantify cholesterol and cholesterol esters1 and to study in vitro lipid deposition on hydrogel and silicone hydrogel contact lenses2.
The enzyme from Sigma has been used in the development of rapid and sensitive galactose oxidase-peroxidase biosensor for galactose detection with prolonged stability. It has been used as a component of a media (MRS agar) plate containing 3,3′,5,5′-tetramethylbenz
HRP readily combines with hydrogen peroxide (H2O2) and the resultant [HRP-H2O2] complex can oxidize a wide variety of hydrogen donors. The optimal pH is 6.0-6.5 and the enzyme is most stable in the pH range of 5.0-9.0. HRP can be conjugated to antibodies by several different methods that include the use of glutaraldehyde, periodate oxidation, disulfide bonds, and also via amino and thiol directed cross-linkers. It is smaller and more stable than the enzyme labels, β-galactosidase and alkaline phosphatase. Hence, it is the most desired label. Also, its glycosylation leads to lower non-specific binding.3 Sodium azide, cyanide, L-cystine, dichromate, ethylenethiourea, hydroxylamine, sulfide, vanadate, p-aminobenzoic acid, as well as Cd2+, Co2+, Cu2+, Fe3+, Mn2+, Ni2+, and Pb2+ ions are found to inhibit the enzyme activity.4
When incubated with a substrate, horseradish peroxidase produces a coloured, fluorimetric, or luminescent derivative of the labeled molecule, allowing quantification. Horseradish peroxidase has been shown to slightly reduce the level of inhibition in a cydAB mutant.
Horseradish peroxidase is isolated from horseradish roots (Amoracia rusticana) and belongs to the ferroprotoporphyrin group of peroxidases. HRP is a single chain polypeptide containing four disulfide bridges. It is a glycoprotein containing 18% carbohydrate. The carbohydrate composition consists of galactose, arabinose, xylose, fucose, mannose, mannosamine, and galactosamine depending upon the specific isozyme. Its molecular weight (~44 kDa) includes the polypeptide chain (33,890 Daltons), hemin plus Ca2+ (~700 Daltons), and carbohydrate (~9,400 Daltons). At least seven isozymes of HRP exist. The isoelectric point for horseradish peroxidase isozymes ranges from 3.0 - 9.0.
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Type VI, essentially salt-free, lyophilized powder, 250-330 units/mg solid (using pyrogallol)
Type II, essentially salt-free, lyophilized powder, 150-250 units/mg solid (using pyrogallol)
Type I, essentially salt-free, lyophilized powder, 50-150 units/mg solid (using pyrogallol)
Type XII, essentially salt-free, lyophilized powder, 250-330 units/mg solid (using pyrogallol)
Certificate of Analysis
Certificate of Origin
Replaces SPABTS02. Procedure updated to conform to current QUMAS formatting guidelines. Refer to CR SOP-DEK-ENZ42.
Keywords: Extinction coefficient
This procedure is for the determination of Peroxidase enzymatic activity using Pyrogallol as the substrate. Products using this method include, but are not limited to, P1709, P2088, P6140, P6782, P81...
Keywords: Enzymology, Extinction coefficient, Gene expression, Size-exclusion chromatography
Reinheitszahl (RZ) is the ratio of absorbance due to hemin (A403, Soret region) to absorbance due to protein (A275). This ratio (A403/A275) is not a measure of enzyme activity. Products using this me...
As a leading supplier of antibodies, Sigma® Life Science is committed to providing researchers the antibody they need with the quality they deserve. Our Antibody Explorer Search Tool provides easy ac...
Keywords: Buffers, Cell biology, Epigenetics, Immunohistochemistry, Immunoprecipitation, Molecular biology, Neuroscience, Western blot
From our library of Related Content, Sigma-Aldrich presents Enzyme Explorer: the most comprehensive source of enzymes, substrates, activators, & inhibitors.
Keywords: Cell culture, Cell disruption, Cell signaling, Diagnostic, Digestions, Drug discovery, Functional genomics, Gene expression, Genomics, Metabolic Pathways, Molecular biology, Neuroscience, Proteomics
The myeloperoxidase product hypochlorous acid generates irreversible high-density lipoprotein receptor inhibitors. Binder V, Ljubojevic S, Haybaeck J, et al. Arterioscler. Thromb. Vasc. Biol. 33(5), 1020-7, (2013)
Nrf2 is not required for epithelial prohibitin-dependent attenuation of experimental colitis. Kathiria AS, Butcher MA, Hansen JM, et al. Am. J. Physiol. Gastrointest. Liver Physiol. 304(10), G885-96, (2013)
A novel benzo[d]imidazole derivate prevents the development of dextran sulfate sodium-induced murine experimental colitis via inhibition of NLRP3 inflammasome. Liu W, Guo W, Wu J, et al. Biochem. Pharmacol. 85(10), 1504-12, (2013)
Ocular inflammation and corneal permeability alteration by benzalkonium chloride in rats: a protective effect of a myosin light chain kinase inhibitor. Droy-Lefaix MT, Bueno L, Caron P, et al. Invest. Ophthalmol. Vis. Sci. 54(4), 2705-10, (2013)
Berberine attenuates bleomycin induced pulmonary toxicity and fibrosis via suppressing NF-κB dependant TGF-β activation: a biphasic experimental study. Chitra P, Saiprasad G, Manikandan R, et al. Toxicol. Lett. 219(2), 178-93, (2013)
Serum phospholipid fatty acids, genetic variation in myeloperoxidase, and prostate cancer risk in heavy smokers: a gene-nutrient interaction in the carotene and retinol efficacy trial. Cheng TY, King IB, Barnett MJ, et al. Am. J. Epidemiol. 177(10), 1106-17, (2013)
Dose- and time-dependent effects of lipopolysaccharide on technetium-99-m-labeled diethylene-triamine pentaacetatic acid clearance, respiratory system mechanics and pulmonary inflammation. Kastis GA, Toumpanakis D, Loverdos K, et al. Exp. Biol. Med. (Maywood.) 238(2), 209-22, (2013)
Design, synthesis, and structure-activity relationship studies of novel 3-alkylindole derivatives as selective and highly potent myeloperoxidase inhibitors. Soubhye J, Aldib I, Elfving B, et al. J. Med. Chem. 56(10), 3943-58, (2013)
Acute aortocaval fistula: role of low perfusion pressure and subendocardial remodeling on left ventricular function. Mazzo FR, de Carvalho Frimm C, Moretti AI, et al. Int. J. Exp. Pathol. 94(3), 178-87, (2013)
Ethyl pyruvate significantly inhibits tumour necrosis factor-α, interleukin-1β and high mobility group box 1 releasing and attenuates sodium taurocholate-induced severe acute pancreatitis associated with acute lung injury. Luan ZG, Zhang J, Yin XH, et al. Clin. Exp. Immunol. 172(3), 417-26, (2013)
Differences in the behavior of advanced glycation end products and advanced oxidation protein products in patients with allergic rhinitis. Di Lorenzo G, Minciullo PL, Leto-Barone MS, et al. J. Investig. Allergol. Clin. Immunol. 23(2), 101-6, (2013)
Differential antioxidative response of tolerant and sensitive maize (Zea mays L.) genotypes to drought stress at reproductive stage. Chugh V, Kaur N, Grewal MS, et al. Indian J. Biochem. Biophys. 50(2), 150-8, (2013)
Pattern recognition receptors and interleukin-8 mediate effects of Gram-positive and Gram-negative bacteria on lung epithelial cell function. Sorrentino R, de Souza PM, Sriskandan S, et al. Br. J. Pharmacol. 154(4), 864-71, (2008)
Metabolic responses to high-fat diets rich in n-3 or n-6 long-chain polyunsaturated fatty acids in mice selected for either high body weight or leanness explain different health outcomes. Nuernberg K, Breier BH, Jayasinghe SN, et al. Nutr. Metab. 8(1), 56, (2011)
Rapid and sensitive galactose oxidase-peroxidase biosensor for galactose detection with prolonged stability Tkác J, et al. Biotechnol. Tech. 13(12), 931-936, (1999)
Lactobacillus species identification, H2O2 production, and antibiotic resistance and correlation with human clinical status. Felten A, Barreau C, Bizet C, et al. J. Clin. Microbiol. 37(3), 729-33, (1999)
Stabilization effect of polyvinyl alcohol on horseradish peroxidase, glucose oxidase, β-galactosidase and alkaline phosphatase. Boyd S, et al. Biotechnol. Tech. 10(9), 693-698, (1996)
Export of functional Streptomyces coelicolor alditol oxidase to the periplasm or cell surface of Escherichia coli and its application in whole-cell biocatalysis. van Bloois E Appl. Microbiol. Biotechnol. 83(4), 679-87, (2009)
3. From Concept to Product Development Enzyme Immunoassay, (1996), 169-171
4. Zollner, H. Handbook of Enzyme Inhibitors 2nd ed., 1993, 367-368
Bergmeyer, H.U., et. al. Methods of Enzymatic Analysis, (1974), 1205-1227
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|D9143||o-Dianisidine, peroxidase substrate|
|A4685||Luminol sodium salt|
|A1888||2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt, ≥98% (HPLC)|
|G5502||Guaiacol, oxidation indicator|
|A3537||5-Aminosalicylic acid, ≥99%|
|A4382||4-Aminoantipyrine, reagent grade|
|A8511||Luminol, ≥97% (HPLC)|
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