Proteinase, bacterial
Type XXIV, 7.0-14.0 units/mg solid, lyophilized powder
DOWNLOAD MSDS (PDF)Synonym: Alkaline Protease, Protease from Bacillus licheniformis, Proteinase from Bacillus licheniformis, Subtilo peptidase A
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CAS Number 9014-01-1
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EC Number 232-752-2
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MDL number MFCD00132092
Properties
| Related Categories | 3.4.x.x Peptidases, 3.x.x.x Hydrolases, Analytical and Industrial Enzymes, Application Index, Biochemicals and Reagents, |
| type | Type XXIV |
| form | lyophilized powder |
| activity | ≤0.05 Kunitz units/mg solid RNase |
| ≤5.0 Kunitz units/mg solid DNase | |
| purified by | crystallization |
| storage temp. | −20°C |
Description
Frequently Asked Questions
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Analysis Note
Amino acid analysis and isoelectric focusing electrophoresis consistent with subtilisin Carlsberg.
Specificity
Subtilisin A is a member of the Serine S8 Endoproteinase family. It has broad specificity with a preference for a large uncharged residue in the P1 position. It hydrolyzes native and denatured proteins, and is active under alkaline conditions.
Unit Definition
One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).
Application
This is a proteolytic enzyme isolated from the fermentation of Bacillus licheniformis. It is a serine endoproteinase with a broad specificity towards native and denatured proteins, and is active under alkaline conditions. Product P8038, also known as Subtilisin Carlsberg, has been used to hydrolyze cardiac cells to study the silencing of cardiac mitochondrial NHE11.
Biochem/physiol Actions
Proteinase catabolizes proteins by hydrolysis of peptide bonds. Proteases are inactivated by serine active-site inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphat
Physical properties
Subtilisin is a non-glycosylated single polypeptide chain without disulfide bonds and has a molecular weight of 27 KDa.
Protocols & Applications
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