|Related Categories||1.11.x.x Peroxide as receptor, 1.x.x.x Oxidoreductases, Analytical and Industrial Enzymes, Application Index, Biochemicals and Reagents,|
|form||essentially salt-free, lyophilized powder|
|absorbance ratio||RZ 2.5-4.0|
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The RZ (Reinheitszahl) is the absorbance ratio A403/A275 determined at 0.5-1.0 mg/ml in deionized water. It is a measure of hemin content, not enzymatic activity. Even preparations with high RZ may have low enzymatic activity.
One pyrogallol unit will form 1.0 mg purpurogallin from pyrogallol in 20 sec at pH 6.0 at 20 °C.
Horseradish peroxidase (HRP) is isolated from horseradish roots (Amoracia rusticana) and belongs to the ferroprotoporphyrin group of peroxidases.. It is used in biochemistry applications such as western blots, ELISA and Immunohistochemistry. Horseradish peroxidase is used to amplify a weak signal and increase detectability of a target molecule, such as a protein. Product P8375, type VI, is an essentially salt free lyophilized powder. It is commonly used to determine amounts of glucose and peroxides in solution. It has been used to study sensory input of cervical spinal cord neurons1.
When incubated with a substrate, horseradish peroxidase produces a coloured, fluorimetric, or luminescent derivative of the labeled molecule, allowing quantification. Horseradish peroxidase has been shown to slightly reduce the level of inhibition in a cydAB mutant. Known inhibitors are sodium azide, cyanide, L-cystine, dichromate, ethylenethiourea, hydroxylamine, sulfide, vanadate, p-aminobenzoic acid, and Cd2+, Co2+, Cu2+, Fe3+, Mn2+, Ni2+, and Pb2+ ions.
HRP is a single chain polypeptide containing four disulfide bridges. It is a glycoprotein containing 18% carbohydrate. The carbohydrate composition consists of galactose, arabinose, xylose, fucose, mannose, mannosamine, and galactosamine depending upon the specific isozyme. Its molecular weight (~44 kDa) includes the polypeptide chain (33,890 Daltons), hemin plus Ca2+ (~700 Daltons), and carbohydrate (~9,400 Daltons). At least seven isozymes of HRP exist. The isoelectric point for horseradish peroxidase isozymes ranges from 3.0 - 9.0.
Horseradish peroxidase is isolated from horseradish roots (Amoracia rusticana) and belongs to the ferroprotoporphyrin group of peroxidases. HRP is a single chain polypeptide containing four disulfide bridges. It is a glycoprotein containing 18% carbohydrate. The carbohydrate composition consists of galactose, arabinose, xylose, fucose, mannose, mannosamine, and galactosamine depending upon the specific isozyme. Its molecular weight (~44 kDa) includes the polypeptide chain (33,890 Daltons), hemin plus Ca2+ (~700 Daltons), and carbohydrate (~9,400 Daltons). At least seven isozymes of HRP exist. The isoelectric point for horseradish peroxidase isozymes ranges from 3.0 - 9.0.
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Type X-S, lyophilized powder, 100,000-250,000 units/g solid (without added oxygen)
Type VI-A, essentially salt-free, lyophilized powder, 950-2000 units/mg solid (using ABTS), 250-330 units/mg solid (using pyrogallol)
Type II, essentially salt-free, lyophilized powder, 150-250 units/mg solid (using pyrogallol)
Type I, essentially salt-free, lyophilized powder, 50-150 units/mg solid (using pyrogallol)
Type XII, essentially salt-free, lyophilized powder, 250-330 units/mg solid (using pyrogallol)
Certificate of Analysis
Certificate of Origin
This procedure is for the determination of Peroxidase enzymatic activity using Pyrogallol as the substrate. Products using this method include, but are not limited to, P1709, P2088, P6140, P6782, P81...
Keywords: Enzymology, Extinction coefficient, Gene expression, Size-exclusion chromatography
Reinheitszahl (RZ) is the ratio of absorbance due to hemin (A403, Soret region) to absorbance due to protein (A275). This ratio (A403/A275) is not a measure of enzyme activity. Products using this me...
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The myeloperoxidase product hypochlorous acid generates irreversible high-density lipoprotein receptor inhibitors. Binder V, Ljubojevic S, Haybaeck J, et al. Arterioscler. Thromb. Vasc. Biol. 33(5), 1020-7, (2013)
Nrf2 is not required for epithelial prohibitin-dependent attenuation of experimental colitis. Kathiria AS, Butcher MA, Hansen JM, et al. Am. J. Physiol. Gastrointest. Liver Physiol. 304(10), G885-96, (2013)
A novel benzo[d]imidazole derivate prevents the development of dextran sulfate sodium-induced murine experimental colitis via inhibition of NLRP3 inflammasome. Liu W, Guo W, Wu J, et al. Biochem. Pharmacol. 85(10), 1504-12, (2013)
Ocular inflammation and corneal permeability alteration by benzalkonium chloride in rats: a protective effect of a myosin light chain kinase inhibitor. Droy-Lefaix MT, Bueno L, Caron P, et al. Invest. Ophthalmol. Vis. Sci. 54(4), 2705-10, (2013)
Berberine attenuates bleomycin induced pulmonary toxicity and fibrosis via suppressing NF-κB dependant TGF-β activation: a biphasic experimental study. Chitra P, Saiprasad G, Manikandan R, et al. Toxicol. Lett. 219(2), 178-93, (2013)
Serum phospholipid fatty acids, genetic variation in myeloperoxidase, and prostate cancer risk in heavy smokers: a gene-nutrient interaction in the carotene and retinol efficacy trial. Cheng TY, King IB, Barnett MJ, et al. Am. J. Epidemiol. 177(10), 1106-17, (2013)
Dose- and time-dependent effects of lipopolysaccharide on technetium-99-m-labeled diethylene-triamine pentaacetatic acid clearance, respiratory system mechanics and pulmonary inflammation. Kastis GA, Toumpanakis D, Loverdos K, et al. Exp. Biol. Med. (Maywood.) 238(2), 209-22, (2013)
Design, synthesis, and structure-activity relationship studies of novel 3-alkylindole derivatives as selective and highly potent myeloperoxidase inhibitors. Soubhye J, Aldib I, Elfving B, et al. J. Med. Chem. 56(10), 3943-58, (2013)
Acute aortocaval fistula: role of low perfusion pressure and subendocardial remodeling on left ventricular function. Mazzo FR, de Carvalho Frimm C, Moretti AI, et al. Int. J. Exp. Pathol. 94(3), 178-87, (2013)
Ethyl pyruvate significantly inhibits tumour necrosis factor-α, interleukin-1β and high mobility group box 1 releasing and attenuates sodium taurocholate-induced severe acute pancreatitis associated with acute lung injury. Luan ZG, Zhang J, Yin XH, et al. Clin. Exp. Immunol. 172(3), 417-26, (2013)
Differences in the behavior of advanced glycation end products and advanced oxidation protein products in patients with allergic rhinitis. Di Lorenzo G, Minciullo PL, Leto-Barone MS, et al. J. Investig. Allergol. Clin. Immunol. 23(2), 101-6, (2013)
[Effect of population density on enzymatic activity of antioxidative and phenol oxidase systems of imagoes and nymphs of the marble cockroach Nauphoeta cinerea]. Murzagulov GS, Saltykova ES, Gaĭfullina LR, et al. Zh. Evol. Biokhim. Fiziol. 49(1), 24-9, (2013)
Differential antioxidative response of tolerant and sensitive maize (Zea mays L.) genotypes to drought stress at reproductive stage. Chugh V, Kaur N, Grewal MS, et al. Indian J. Biochem. Biophys. 50(2), 150-8, (2013)
Pattern recognition receptors and interleukin-8 mediate effects of Gram-positive and Gram-negative bacteria on lung epithelial cell function. Sorrentino R, de Souza PM, Sriskandan S, et al. Br. J. Pharmacol. 154(4), 864-71, (2008)
Metabolic responses to high-fat diets rich in n-3 or n-6 long-chain polyunsaturated fatty acids in mice selected for either high body weight or leanness explain different health outcomes. Nuernberg K, Breier BH, Jayasinghe SN, et al. Nutr. Metab. 8(1), 56, (2011)
Export of functional Streptomyces coelicolor alditol oxidase to the periplasm or cell surface of Escherichia coli and its application in whole-cell biocatalysis. van Bloois E Appl. Microbiol. Biotechnol. 83(4), 679-87, (2009)
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|D9143||o-Dianisidine, peroxidase substrate|
|A4685||Luminol sodium salt|
|A1888||2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt, ≥98% (HPLC)|
|G5502||Guaiacol, oxidation indicator|
|A3537||5-Aminosalicylic acid, ≥99%|
|A4382||4-Aminoantipyrine, reagent grade|
|A8511||Luminol, ≥97% (HPLC)|
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