Synonym: Pancreatic Ribonuclease, RNase A, Ribonuclease I, Ribonucleate 3′-pyrimidinooligonucleotidohydrolase
|Related Categories||3.1.x.x Acting on esters, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Core Bioreagents,|
|grade||for molecular biology|
|form||salt-free, lyophilized powder|
|mol wt||mol wt ~13,700|
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Protein determined by E
Boiling of stock solutions to inactivate residual DNase is not necessary or recommended.
• RNase protection assays
• Remove unspecifically bound RNA
• Analysis of RNA sequences
• Hydrolyze RNA contained in protein samples
• Purification of DNA
Derived from New Zealand-sourced pancreas.
Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. It is also used in RNA sequence analysis and protection assays.
Tested in plasmid purification. At concentrations up to 10 μg per mL, nicking or degradation of plasmid is not detectable. Detection limit: Degradation of 10% of the DNA.
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Certificate of Analysis
Certificate of Origin
1. OBJECTIVE To standardize a procedure for the enzymatic assay of Ribonuclease A in Analytical Services at Sigma-Aldrich St. Louis.
Keywords: Sample preparations
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|D5758||Diethyl pyrocarbonate, ≥97% (NMR)|
|R4642||Ribonuclease A from bovine pancreas, ≥70 Kunitz units/mg protein, (Solution of 50% glycerol, 10mM Tris-HCL pH 8.0)|
|R2520||Ribonuclease inhibitor human, Inhibits ribonucleases during transcription and translation experiments|
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