Synonym: Pancreatic Ribonuclease, RNase A, Ribonuclease I, Ribonucleate 3′-pyrimidinooligonucleotidohydrolase
CAS Number 9001-99-4
EC Number 232-646-6
MDL number MFCD00132181
Popular Documents: Datasheet (PDF) | Specification Sheet (PDF)
| Related Categories | 3.1.x.x Acting on esters, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Core Bioreagents, |
| grade | for molecular biology |
| type | Type I-AS |
| form | salt-free, lyophilized powder |
| mol wt | mol wt ~13,700 |
| purified by | chromatography |
| storage temp. | −20°C |
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Protein determined by E
Boiling of stock solutions to inactivate residual DNase is not necessary or recommended.
• RNase protection assays
• Remove unspecifically bound RNA
• Analysis of RNA sequences
• Hydrolyze RNA contained in protein samples
• Purification of DNA
Derived from New Zealand-sourced pancreas.
Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. It is also used in RNA sequence analysis and protection assays.
Tested in plasmid purification. At concentrations up to 10 μg per mL, nicking or degradation of plasmid is not detectable. Detection limit: Degradation of 10% of the DNA.
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