R6513 Sigma

Ribonuclease A from bovine pancreas

≥70 Kunitz units/mg protein, salt-free, lyophilized powder

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Synonym: Pancreatic Ribonuclease, RNase A, Ribonuclease I, Ribonucleate 3′-pyrimidinooligonucleotidohydrolase

Description

Frequently Asked Questions

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Analysis Note

Protein determined by E1%/280.

Caution

Boiling of stock solutions to inactivate residual DNase is not necessary or recommended.

Features and Benefits

• RNase protection assays
• Remove unspecifically bound RNA
• Analysis of RNA sequences
• Hydrolyze RNA contained in protein samples
• Purification of DNA

Preparation Note

Derived from New Zealand-sourced pancreas.

Application

Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. It is also used in RNA sequence analysis and protection assays.

Tested in plasmid purification. At concentrations up to 10 μg per mL, nicking or degradation of plasmid is not detectable. Detection limit: Degradation of 10% of the DNA.

Price and Availability

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