R7388 Sigma

RPMI-1640 Medium

Modified, with 20 mM HEPES and L-glutamine, without sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

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Properties

Related Categories Cell Culture, Classic Media and Salts, RPMI- 1640 Medium More...
Quality Level   GMP
sterility   sterile-filtered
form   liquid
impurities   endotoxin, tested
pH range   >7.2
suitability   suitable for cell culture
storage temp.   2-8°C

Description

Application

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells.

Protocols & Applications

ECACC Handbook-Fundamental Techniques in Cell Culture

Cryostor Cryopreservation Video Protocol

Mycoplasma PCR Detection Kit Video Protocol

Price and Availability

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