S5933 Sigma

Anti-STAT3 antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies against Proteins/Bioactives/Markers/Receptors for Stem Cell Biology, Antibodies for Cell Biology, Antibodies for Embryonic Stem Cells,
species reactivity   mouse, rat, human
application(s)   immunocytochemistry: 10 μg/mL using A431 cells
  immunoprecipitation: 4 μg using STAT3 from 0.5–1 mg of an EGF stimulated human A431 cell lysate
  western blot (chemiluminescent): 0.5-2 μg/mL using lysates from EGF stimulated human A431, mouse WEHI and rat L6 cells
clone   polyclonal
antibody form   IgG fraction of antiserum
form   buffered aqueous solution
mol wt   antigen mol wt 92 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... STAT3(6774)
mouse ... Stat3(20848)
rat ... Stat3(25125)

Description

Immunogen

GST fusion protein corresponding to amino acids 688-722 of human STAT3, expressed in bacteria.

Physical form

Solution in 0.1 M Tris-glycine, pH 7.4, containing 0.15 M NaCl and 0.05% sodium azide.

Specificity

The antibody is specific for STAT3. It may detect an additional 66 kDa band using high concentrations or long exposures. The sequence recognized by the antibody (688-722) is identical in mouse and rat STAT3. The first 28 amino acids are identical to mouse STAT3B.

Price and Availability

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