|Related Categories||Bioactive Small Molecules, Cell Biology, Cell Signaling and Neuroscience, D-DIF, Kinase/Phosphatase Biology,|
|solubility||H2O: soluble1 mM|
|DMSO: freely soluble|
|ethanol: freely soluble|
|shipped in||dry ice|
Selective cAMP-dependent protein kinase (PKA) activator. Especially suitable for intact cells showing preference for site B of type II isozyme. Completely stable against mammalian cPDE types I & III and only extremely slowly hydrolized by cPDE type II. Surpasses the widely used but problematic dibutyryl cAMP or 8-CPT-cAMP in terms of metabolic stability, membrane permeability and potency.
Sp 5,6-DCl-cBIMPS may be used in cAMP-dependent protein kinase-mediated cell signaling studies.
Customers Also Viewed
≥97.0% (HPLC), powder
≥99% (HPLC), solid
99 atom % D
≥98% (HPLC), solid
Cyclic AMP-dependent protein kinase (PKA or cAK) and cyclic GMP-dependent protein kinase (PKG or cGK) transfer the γ-phosphate of ATP to serine and threonine residues of many cellular proteins. PKAs ...
Analysis and regulation of vasodilator-stimulated phosphoprotein serine 239 phosphorylation in vitro and in intact cells using a phosphospecific monoclonal antibody. Smolenski A, Bachmann C, Reinhard K, et al. J. Biol. Chem. 273(32), 20029-35, (1998)
Relationship between cyclic AMP-dependent protein tyrosine phosphorylation and extracellular calcium during hyperactivation of boar spermatozoa. Harayama H, Noda T, Ishikawa S, et al. Mol. Reprod. Dev. 79(10), 727-39, (2012)
Guanine-nucleotide exchange factors (RAPGEF3/RAPGEF4) induce sperm membrane depolarization and acrosomal exocytosis in capacitated stallion sperm. McPartlin LA, Visconti PE, and Bedford-Guaus SJ Biol. Reprod. 85(1), 179-88, (2011)
Hyperactivated motility of frozen-thawed spermatozoa from fertile and subfertile Japanese black bulls induced by cyclic adenosine 3',5'-monophosphate analogue, cBiMPS. Murase T, El-Kon I, Harayama H, et al. J. Reprod. Dev. 56(1), 36-40, (2010)
Calyculin A-sensitive protein phosphatases are involved in maintenance of progressive movement in mouse spermatozoa in vitro by suppression of autophosphorylation of protein kinase A. Goto N and Harayama H J. Reprod. Dev. 55(3), 327-34, (2009)
Effects of protein phosphatase inhibitor calyculin a on the postacrosomal protein serine/threonine phosphorylation state and acrosome reaction in boar spermatozoa incubated with a cAMP analog. Adachi J, Tate S, Miyake M, et al. J. Reprod. Dev. 54(3), 171-6, (2008)
Changes of PKA and PDK1 in the principal piece of boar spermatozoa treated with a cell-permeable cAMP analog to induce flagellar hyperactivation. Harayama H and Nakamura K Mol. Reprod. Dev. 75(9), 1396-407, (2008)
Urocortin-induced decrease in Ca2+ sensitivity of contraction in mouse tail arteries is attributable to cAMP-dependent dephosphorylation of MYPT1 and activation of myosin light chain phosphatase. Lubomirov LT, Reimann K, Metzler D, et al. Circ. Res. 98(9), 1159-67, (2006)
A cyclic adenosine 3',5'-monophosphate stimulates phospholipase Cgamma1-calcium signaling via the activation of tyrosine kinase in boar spermatozoa. Harayama H, Murase T, and Miyake M J. Androl. 26(6), 732-40, (2005)
Regulation of aldosterone production from zona glomerulosa cells by ANG II and cAMP: evidence for PKA-independent activation of CaMK by cAMP. Gambaryan S, Butt E, Tas P, et al. Am. J. Physiol. Endocrinol. Metab. 290(3), E423-33, (2006)
Viability and protein phosphorylation patterns of boar spermatozoa agglutinated by treatment with a cell-permeable cyclic adenosine 3',5'-monophosphate analog. Harayama H J. Androl. 24(6), 831-42, (2003)
Involvement of cytoplasmic free calcium in boar sperm: head-to-head agglutination induced by a cell-permeable cyclic adenosine monophosphate analog. Harayama H, Okada K, and Miyake M J. Androl. 24(1), 91-9, (2003)
Bicarbonate stimulation of boar sperm motility via a protein kinase A-dependent pathway: between-cell and between-ejaculate differences are not due to deficiencies in protein kinase A activation. Holt WV and Harrison RA J. Androl. 23(4), 557-65, (2002)
Two critical periods for cAMP-dependent protein kinase activity during long-term memory consolidation in the crab Chasmagnathus. Locatelli F, Maldonado H, and Romano A Neurobiol. Learn. Mem. 77(2), 234-49, (2002)
Urocortin relaxes rat tail arteries by a PKA-mediated reduction of the sensitivity of the contractile apparatus for calcium. Lubomirov L, Gagov H, Petkova-Kirova P, et al. Br. J. Pharmacol. 134(7), 1564-70, (2001)
Characterization of Sp-5,6-dichloro-1-β-D-ribofuranosylbenzimidazole-3',5'-monophosphorothioate (Sp-5,6-DCl-cBiMPS) as a potent and specific activator of cyclic-AMP-dependent protein kinase in cell extracts and intact cells. Sandberg, M., et. al. Biochem. J. 279, 521-527, (1991)
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Need larger quantities for your development, manufacturing or research applications?