|Related Categories||Biochemicals and Reagents, Core Bioreagents, Fluorescent Kits, Fluorescent Probes, Labels, Particles and Stains, Life Science Reagents for PCR,|
|concentration||1.25 units/reaction (50 μL reaction volume)|
|shipped in||wet ice|
• SYBR Green I dye binds to double-stranded DNA and is ideal for quantifying any DNA sequence. Detection is monitored by measuring the increase in fluorescence throughout cycling.
• JumpStart Taq DNA polymerase prevents amplification of non-specific products while increasing target yield.
• Internal Reference Dye is provided for reaction normalization. Maximum excitation and emission is 586 nm and 605 nm, respectively.
• SYBR Green JumpStart Taq ReadyMix reduces preparation time and the risk of contamination from multiple pipetting steps.
SYBR Green ReadyMix for High Throughput Quantitative PCR combines the performance enhancements of JumpStart Taq and SYBR Green I in an easy-to-use ReadyMix solution that incorporates ROX dye for ABI and other real time instrument applications. The ReadyMix includes a detection fluor, internal standard and reagents for PCR making it the ideal solution for performing high-throughput quantitative PCR.
Default reaction volume is 50 μL
20RXN is packaged as 1 X 500 μL
400RXN is packaged as 1 X 10 mL
2000RXN is packaged as 1 X 50 mL
Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,994,056 and 6,171,785.. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim (such as apparatus or system claims in US Patent No. 6,814,934) and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
JumpStart is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
SYBR is a registered trademark of Life Technologies
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The highly variable regions within the 16S ribosomal RNA (rRNA) gene sequences are being exploited with PCR and metagenomic sequencing to characterize the microbial strains within the gastrointestina...
Biofiles Edition 6.2
Keywords: Gastrointestinal, Nucleic acid hybridization, Polymerase chain reaction, Polymerase chain reaction - quantitative, Probiotics, Sequencing
Sambrook, J., and Russell, D.W. Molecular Cloning: A Laboratory Manual
Validation of Quantitative PCR Assays Lovatt, A., et al. BioPharm. March, 22-23, (2002)
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|Z702439||A-Z of Quantitative PCR, lots of recipes from distinguished contributors|
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