MISSION® pLKO.1-puro Empty Vector Control Plasmid DNA
Contains no shRNA insert
DOWNLOAD MSDS (PDF)Synonym: MISSION® Control Vectors
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MDL number MFCD07785395
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Popular Documents: Bulletin (PDF)
Properties
| Related Categories | Cell Biology, Functional Genomics and RNAi, General Stem Cell Biology, MISSION Controls in DNA Format, MISSION shRNA, |
| shipped in | dry ice |
| storage temp. | −20°C |
Description
Frequently Asked Questions
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Application
To see more application data, protocols, vector maps visit sigma.com/shrna.
General description
The MISSION pLKO.1-puro Control Vector is a lentivirus plasmid vector. The vector does not contain an shRNA insert and is useful as a negative control in experiments using the MISSION shRNA library clones. This allows one to examine the effect of transfection on gene expression and interpret the knockdown effect seen with shRNA clones. Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. The pLKO.1-puro Control Vector is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl.
Legal Information
Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/m
MISSION is a registered trademark of Sigma-Aldrich Co. LLC
Price and Availability
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