TNF-α human
Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), cell culture tested
DOWNLOAD MSDS (PDF)Synonym:
Cachectin,
Cytotoxin,
Differentiation-
Properties
| Related Categories | Cancer Research, Cell Biology, Cell Culture, Cell Signaling and Neuroscience, Core Bioreagents, |
| recombinant | expressed in E. coli |
| assay | ≥98% (HPLC) |
| ≥98% (SDS-PAGE) | |
| form | lyophilized |
| mol wt | mol wt 17.4 kDa |
| impurities | endotoxin, tested |
| suitability | cell culture tested |
Description
Biochem/physiol Actions
TNF-α is a pleiotropic pro-inflammatory cytokine secreted by various cells including adipocytes, activated monocytes, macrophages, B cells, T cells and fibroblasts. Recombinant human TNF-a is a soluble 157 amino acid protein (17.4 kDa) which corresponds to C-terminal extracellular domain of the full length transmembrane protein.
Reconstitution
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a stabilizer (example 5% Trehalose) and store in working aliquots at -20°C to -80°C.
Physical form
Lyophilized from 10 mM Sodium Phosphate, pH 7.4 + 0.5mM DTT.
General description
TNF-α is a pleiotropic pro-inflammatory cytokine secreted by various cells including adipocytes, activated monocytes, macrophages, B cells, T cells and fibroblasts. It belongs to the TNF family of ligands and signals through two receptors, TNFR1 and TNFR2. TNF-a is cytotoxic to a wide variety of tumor cells and is an essential factor in mediating the immune response against bacterial infections. TNF-a also plays a role in the induction of septic shock, auto immune diseases, rheumatoid arthritis, inflammation, and diabetes. Recombinant human TNF-a is a soluble 157 amino acid protein (17.4 kDa) which corresponds to C-terminal extracellular domain of the full length transmembrane protein.
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