Trypsin-EDTA solution
1 ×, sterile; sterile-filtered, BioReagent, suitable for cell culture, 0.5 g porcine trypsin and 0.2 g EDTA • 4Na per liter of Hanks′ Balanced Salt Solution with phenol red
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MDL number MFCD00130286
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Properties
| Related Categories | Analytical and Industrial Enzymes, Application Index, Biochemicals and Reagents, Biomolecular Mass Spectrometry, Cell Culture, |
| Quality Level | GMP |
| sterility | sterile; sterile-filtered |
| product line | BioReagent |
| concentration | 1 × |
| impurities | Porcine parvovirus, none detected (9 CFR) |
| pH-range | 7.0 - 7.6 |
| suitability | suitable for cell culture |
Description
Application
Trypsin-EDTA solutions are used to release adherent cells from culture surfaces. They are generally composed of variable concentrations of natural porcine pancreas-derived trypsin and EDTA. Trypsin 1X solutions can range from 0.025% to 0.5%. The reasons for the range of concentrations are differences in required trypsin activity or potency; differences in incubation times; and cell line susceptibility. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture. Various formulations should be tested to determine the best product for a specific application.
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