Tris-Glycine Buffer 10× Concentrate
DOWNLOAD MSDS (PDF)
-
Popular Documents: Specification Sheet (PDF)
Properties
| Related Categories | Acrylamides, Biological Buffers, Buffer / Buffer Salts, Buffer Convenience Packaging, Buffer Solutions, |
| sterility | 0.2 μm filtered |
| foreign activity | Protease, none detected |
| storage temp. | 2-8°C |
Description
Application
For Western blotting and gel electrophoresis. Tris-glycine buffer is used to make a Tris-glycine-methanol transfer buffer, which is the most common protein transfer buffer for wet blot transfers. The methanol prevents the gel from swelling during the transfer and enhances the protein binding to nitrocellulose.The 10× Tris-glycine buffer is diluted to 1× with methanol and water to make a solution containing 25 mM Tris, 192 mM glycine, and 20% methanol. A sufficient amount of transfer buffer should be made to cover the electrode wires in the wet blot transfer unit and to soak the gel, membrane and blotting paper.
Other Notes
0.25 M Tris, 1.92 M glycine, pH approx. 8.3
Contains no methanol.
Price and Availability
Customers Also Viewed
10 ×, solution
BioReagent, for molecular biology, 10x concentrate
BioUltra, 10× concentrate
Technical Service:
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Bulk Ordering & Pricing:
Need larger quantities for your development, manufacturing or research applications?
