|Related Categories||Biochemicals, Core Bioreagents, Life Science Reagents for DNA/RNA Electrophoresis, Molecular Biology, Molecular Biology Reagents,|
|measuring range||<250 ng/band detection sensitivity|
BlueView is a quick, safe alternative to ethidium bromide staining for nucleic acid electrophoresis. BlueView can be used directly as the running buffer and in the gel for instant staining of the bands of nucleic acids during electrophoresis, visible in ambient light. Bands can be excised from the gel without the use of UV which can cause nicking. BlueView stained bands can be used for PCR, ligation, labeling, restriction digestion, and Southern blotting.
Reconstitution with water produces a 10× stock solution of the BlueView stain in 10× TAE or TBE buffer. The bottles are large enough to contain the concentrate. Dilute the 10× stock solution to the desired concentration before use. Concentrations of TBE and TAE between 0.5 and 1× are commonly used for nucleic acid electrophoresis. A 20-fold dilution of the stock solution is recommended for low background staining and optimum signal to noise.
BlueView is a trademark of Sigma-Aldrich Co. LLC
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Certificate of Analysis
|Precautionary statements||P261-P305 + P351 + P338|
|Personal Protective Equipment||dust mask type N95 (US), Eyeshields, Gloves|
|Hazard Codes (Europe)||Xi|
|Risk Statements (Europe)||36/37/38|
|Safety Statements (Europe)||26|
Reaction Optimization Reliable amplification of long DNA sequences requires: 1) effective denaturation of DNA template, 2) adequate extension times to produce large products and 3) protection of targ...
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