Serum Triglyceride Determination Kit

Frequently Asked Questions

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Linkage

In addition to kits, the individual reagents and glycerol standard are available separately when fewer reactions are needed.

Packaging

The kit also includes sufficient reagent for an additional 250 free glyceride tests for true triglyceride determination.

Application

A Serum Triglyceride Determination Kit was used to determine the level of triglycerides in whole-body homogenates of Drosophila melanogaster. ¹

The Serum Triglyceride Determination Kit can be used for the measurement of glycerol, true triglycerides, or total triglycerides in serum or plasma. The procedure involves enzymatic hydrolysis by lipase of the triglycerides to glycerol and free fatty acids. The glycerol produced is then measured by coupled enzyme reactions. Many of the triglyceride reagents which are commercially available, do not differentiate between endogenous glycerol and glycerol derived by hydrolytic action of lipase on glycerides.
Triglycerides are first hydrolyzed by lipoprotein lipase to glycerol and free fatty acids. Glycerol is then phosphorylated by adenosine-5′-triphosphate (ATP) forming glycerol-1-phosphate (G-1-P) and adenosine-5′-diphosphate (ADP) in the reaction catalyzed by glycerol kinase (GK). G-1-P is then oxidized by glycerol phosphate oxidase (GPO) to dihydroxy-acetone phosphate (DAP) and hydrogen peroxide (H₂O₂). Peroxidase (POD) catalyzes the coupling of H₂O₂ with 4-aminoantipyrine (4-AAP) and sodium
N-ethyl-N-(3-sulfopropyl) m-anisidine (ESPA) to produce a quinoneimine dye that shows an absorbance maximum at 540 nm. The increase in absorbance at 540 nm is directly proportional to triglyceride concentration of the sample.

The triglyceride and free glycerol kits are for the quantitative determination of glycerol, total triglycerides or free triglycerides.