Synonym: XOD, Xanthine:oxygen oxidoreductase
|Related Categories||1.1.x.x Acting on hydroxyl groups, 1.x.x.x Oxidoreductases, Application Index, Biochemicals and Reagents, Enzyme Class Index,|
|mol wt||mol wt ~160 kDa|
|Gene Information||Escherichia coli K12 ... xdhA(947116), xdhB(947205), xdhC(945148)|
This enzyme is useful for enzymatic determination of inorganic phosphorus, 5′-nucleotidase and adenosine deaminase when coupled with Purine-nucleoside phosphorylase (PNP-301) and uricase (UAO-201, UAO-211).
Also contains proteolytic activity.
One unit will convert 1.0 μmole of xanthine to uric acid per min at pH 7.5 at 25 °C. Approx. 50% of the activity is obtained with hypoxanthine as substrate.
Lyophilized powder containing BSA and sodium glutamate as stabilizers
Allopurinol is a synthetic drug show to inhibit xanthine oxidase. 1
Inhibitors: Reducing agents, Hg++, Ag+,MIA
Optimum pH: 7.5 - 8.0
Optimum temperature: 65oC
pH Stability: pH 6.5 - 9.0 (25oC, 15hr)
Thermal stability: below 55oC (pH 8.0, 30min)
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Certificate of Analysis
Certificate of Origin
One unit causes the formation of one micromole of uric acid per minute under the conditions described below.
Keywords: Extinction coefficient
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