Xanthine Oxidase microbial lyophilized powder, ≥7 units/mg solid

Properties

Related Categories	1.1.x.x Acting on hydroxyl groups, 1.x.x.x Oxidoreductases, Application Index, Biochemicals and Reagents, Enzyme Class Index, Enzymes for Diagnostic Kit Manufacturing, Enzymes, Inhibitors, and Substrates, General Metabolic Enzymes, Metabolomics, Nucleic Acid Metabolism More...
form	lyophilized powder
mol wt	mol wt ~160 kDa
storage temp.	−20°C
Gene Information	Escherichia coli K12 ... xdhA(947116), xdhB(947205), xdhC(945148)

Description

Application

This enzyme is useful for enzymatic determination of inorganic phosphorus, 5′-nucleotidase and adenosine deaminase when coupled with Purine-nucleoside phosphorylase (PNP-301) and uricase (UAO-201, UAO-211).

Biochem/physiol Actions

Also contains proteolytic activity.

Unit Definition

One unit will convert 1.0 μmole of xanthine to uric acid per min at pH 7.5 at 25 °C. Approx. 50% of the activity is obtained with hypoxanthine as substrate.

Physical form

Lyophilized powder containing BSA and sodium glutamate as stabilizers

General description

Allopurinol is a synthetic drug show to inhibit xanthine oxidase. ¹

Physical properties

Inhibitors: Reducing agents, Hg⁺⁺, Ag⁺,MIA
Optimum pH: 7.5 - 8.0
Optimum temperature: 65^oC
pH Stability: pH 6.5 - 9.0 (25^oC, 15hr)
Thermal stability: below 55^oC (pH 8.0, 30min)