|Related Categories||Automation Reagents, Cell Biology, DNA & RNA Purification, General Stem Cell Biology, Genomic DNA Amplification,|
|usage||sufficient for 1000 amplifications|
|sufficient for 1000 extractions|
|shipped in||wet ice|
For additional information, please see www.sigma-aldrich.com/ext
Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patent rights (such as 5′ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries.
Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
• Efficient 8 minute prep allows greater speed and throughput
• No need for any type of purification, organic extraction, centrifugation or alcohol precipitation
• Simple, 3 step procedure with no special equipment required
• Hot start antibody included for highly specific PCR amplification of genomic DNA
• Compatible with multiple format (single tube or 96-well plate)
• Can be used with whole blood or blood cards
• Extract stable at 4 °C for at least 6 months (Fig. 2)
Genomic DNA is extracted from 10 μl of whole blood by simply adding the Extraction Solution and incubating for 5 minutes at room temperature. The Neutralization Solution is added to the extract to counteract inhibitory substances prior to PCR. A portion of the DNA extract is then added to the specially formulated PCR ReadyMix.
The Extract-N-Amp Blood PCR Kits contain all of the reagents necessary to rapidly extract host genomic DNA from whole blood and amplify targets of interest by PCR (Fig. 1). This novel extraction system eliminates the need for any type of purification, organic extraction, centrifugation, heating, filtration or alcohol precipitation. The kit also includes a PCR ReadyMix™, specially formulated for amplification directly from the extract. This formulation uses an antibody based hot start, for specific amplification.
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Certificate of Analysis
Certificate of Origin
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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