|Related Categories||Cloning and Expression, Core Bioreagents, General Reagents, Life Science Reagents for Cloning, Molecular Biology,|
|grade||for molecular biology|
|usage||1 kit sufficient for >100 standard transformations|
|shipped in||dry ice|
The Yeast Transformation Kit contains:
• 10 μg Control yeast plasmid DNA, pRS316
• 100 mL PLATE buffer
• 100 mL Transformation buffer
Suitable for transformation of any strain of yeast. Convenient, flexible and sensitive, positive transformants can be obtained with as little as 10 ng of DNA; the optimum efficiency is in the 0.1- 3 μg range.
• Easy and ready-to-use
• Requires as little as 10 ng of plasmid DNA
• Flexibility for any strain of yeast
• Sufficient for over 100 standard transformations
Sigma’s Yeast Transformation Kit contains all necessary reagents and controls for efficient transformation of yeast by the lithium acetate method.
Transformation with a plasmid complementing the mutated gene enables the transformant to grow on medium lacking the required component. Yeast cells are made competent for transformation by incubation in a buffered lithium acetate solution. Transformation is then carried out by incubating the cells together with transforming DNA and carrier DNA in a solution containing polyethylene glycol (PEG).
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