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YEAST1 Sigma

Yeast Transformation Kit

reagents for introducing plasmid DNA into yeast


Related Categories Cloning and Expression, Core Bioreagents, General Reagents, Life Science Reagents for Cloning, Molecular Biology,
grade   for molecular biology
usage   1 kit sufficient for >100 standard transformations
shipped in   dry ice
storage temp.   −20°C



The Yeast Transformation Kit contains:
• Transformation Buffer 100 ml, Product Code T0809 (contains 100 mM lithium acetate, 10 mM TrisHCl, pH 7.6, and 1 mM EDTA)
• Plate Buffer 100 ml, Product Code P8966 (contains 40% PEG, 100 mM lithium acetate, 10 mM TrisHCl, pH 7.5, 1 mM EDTA)
• Deoxyribonucleic acid from salmon testes, 10 mg/ml, 2 x 1 ml, Product Code D9156
• Control Yeast Plasmid DNA pRS316 carrying the ura gene, 10 μg, Product Code C4959
• Yeast Synthetic Drop-out Medium Supplement Without Uracil, 1 g, Product Code Y1501


Suitable for transformation of any strain of yeast. Convenient, flexible and sensitive, positive transformants can be obtained with as little as 10 ng of DNA; the optimum efficiency is in the 0.1- 3 μg range.

Features and Benefits

• Easy and ready-to-use
• Requires as little as 10 ng of plasmid DNA
• Flexibility for any strain of yeast
• Sufficient for over 100 standard transformations

General description

Sigma’s Yeast Transformation Kit contains all necessary reagents and controls for efficient transformation of yeast by the lithium acetate method.


Transformation with a plasmid complementing the mutated gene enables the transformant to grow on medium lacking the required component. Yeast cells are made competent for transformation by incubation in a buffered lithium acetate solution. Transformation is then carried out by incubating the cells together with transforming DNA and carrier DNA in a solution containing polyethylene glycol (PEG).

Price and Availability

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