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YEAST1 Sigma

Yeast Transformation Kit

reagents for introducing plasmid DNA into yeast

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Properties

Related Categories Cloning and Expression, Core Bioreagents, General Reagents, Life Science Reagents for Cloning, Molecular Biology,
grade   for molecular biology
usage    kit sufficient for >100 standard transformations
shipped in   dry ice
storage temp.   −20°C

Description

Components

The Yeast Transformation Kit contains:
• Transformation Buffer; 100 ml; 100 mM lithium acetate, 10 mM Tris HCl, pH 7.6, and 1 mM EDTA
• Plate Buffer; 100 ml; 40% PEG, 100 mM lithium acetate, 10 mM Tris HCl, pH 7.5, 1 mM EDTA
• Deoxyribonucleic acid from salmon teste, 10 mg/ml; 2 x 1 ml
• Control Yeast Plasmid DNA pRS316 carrying the ura gene; 10 μg
• Yeast Synthetic Drop-out Medium Supplement Without Uracil; 1 g

Application

Suitable for transformation of any strain of yeast. Convenient, flexible and sensitive, positive transformants can be obtained with as little as 10 ng of DNA; the optimum efficiency is in the 0.1- 3 μg range.

Features and Benefits

• Easy and ready-to-use
• Requires as little as 10 ng of plasmid DNA
• Flexibility for any strain of yeast
• Sufficient for over 100 standard transformations

General description

Sigma’s Yeast Transformation Kit contains all necessary reagents and controls for efficient transformation of yeast by the lithium acetate method.

Principle

Transformation with a plasmid complementing the mutated gene enables the transformant to grow on medium lacking the required component. Yeast cells are made competent for transformation by incubation in a buffered lithium acetate solution. Transformation is then carried out by incubating the cells together with transforming DNA and carrier DNA in a solution containing polyethylene glycol (PEG).

Price and Availability


Oxford Genetics

Synthetic Biology Resource Collection

Kit component also available separately

Description

Product #

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Deoxyribonucleic acid, single stranded from salmon testes, For hybridization 2 x 1 mL MSDS D9156
Yeast Synthetic Drop-out Medium Supplements, without uracil 1 g MSDS Y1501

Kit component only

Description

Product #

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Transformation Buffer 100 mL MSDS    
Plate Buffer 100 mL MSDS    
pRs316 Control plasmid 10 μg MSDS    

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Safety & Documentation

Safety Information

Symbol 
GHS05  GHS05
Signal word 
Danger
Hazard statements 
Precautionary statements 
Hazard Codes (Europe) 
Xi
Risk Statements (Europe) 
41
Safety Statements (Europe) 
26-39
RIDADR 
UN 3316 9

Protocols & Articles

Articles

Protein Expression Systems

The development of genetic engineering and cloning has opened many possibilities of expression and isolation of heterologous proteins for research purposes. Considerable advances in technology have e...
Keywords: Amplification, Antibiotics, Cell disruption, Cloning, Culture media, Gene expression, Genetic, Genetics, Glycosylations, Molecular biology, Purification, Pyrogens, Transfection, transformation

Protocols

Introduction to Yeast Transformation

Transformation is the process by which exogenous DNA is introduced into a cell, resulting in an inheritable change or genetic modification. This was first reported in Streptococcus pneumoniae by Grif...
Keywords: Cell disruption, Food & Beverage, Gene expression, Genetic, Molecular biology, Pharmaceutical, Polymerase chain reaction, transformation

Yeast Transformation Protocols

Yeasts are considered model systems for eukaryotic studies as they exhibit fast growth and have dispersed cells. Moreover, replica plating and mutant isolation of yeast cells can be done with relativ...
Keywords: Degradations, Genetic, Molecular biology, Transfection, transformation

Peer-Reviewed Papers

References

Set your institution to view full text papers.

Altering the laccase functionality by in vivo assembly of mutant libraries with different mutational spectra. Zumárraga M, Camarero S, Shleev S, et al. Proteins 71(1), 250-60, (2008)

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In vitro evolution of a fungal laccase in high concentrations of organic cosolvents. Zumárraga M, Bulter T, Shleev S, et al. Chem. Biol. 14(9), 1052-64, (2007)

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DMSO-enhanced whole cell yeast transformation. Hill, J., et al. Nucleic Acids Res. 19, 5791, (1991)

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Improved method for high efficiency transformation of yeast. Gietz, D., et al. Nucleic Acids Res. 20, 1425, (1992)

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A simple efficient procedure for transformation of yeast. Elble, R. Biotechniques 13, 18-20, (1992)

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Uncoupling antisense-mediated silencing and DNA methylation in the imprinted Gnas cluster. Williamson CM, Ball ST, Dawson C, et al. PLoS Genet. 7(3), e1001347, (2011)

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Transformation of intact yeast cells treated with alkali cations. Ito, H., et al. J. Bacteriol. 153, 163-168, (1983)

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Engineering platforms for directed evolution of Laccase from Pycnoporus cinnabarinus. Camarero S, Pardo I, Cañas AI, et al. Appl. Environ. Microbiol. 78(5), 1370-84, (2012)

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Recruiting a new substrate for triacylglycerol synthesis in plants: the monoacylglycerol acyltransferase pathway. Petrie JR, Vanhercke T, Shrestha P, et al. PLoS ONE 7(4), e35214, (2012)

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Semi-rational engineering of cellobiose dehydrogenase for improved hydrogen peroxide production. Sygmund C, Santner P, Krondorfer I, et al. Microb. Cell Fact. 12, 38, (2013)

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Yeast expression of the Tuber borchii fruiting body specific protein, TBF-1: identification of a noncanonical signal peptide. Palma F, Cerigini E, and Stocchi V FEMS Microbiol. Lett. 272(1), 114-9, (2007)

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