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YEAST1 Sigma

Yeast Transformation Kit

reagents for introducing plasmid DNA into yeast

Properties

Related Categories Cloning and Expression, Core Bioreagents, General Reagents, Life Science Reagents for Cloning, Molecular Biology,
grade   for molecular biology
usage    kit sufficient for >100 standard transformations
shipped in   dry ice
storage temp.   −20°C

Description

Components

The Yeast Transformation Kit contains:
• Transformation Buffer; 100 ml; 100 mM lithium acetate, 10 mM Tris HCl, pH 7.6, and 1 mM EDTA
• Plate Buffer; 100 ml; 40% PEG, 100 mM lithium acetate, 10 mM Tris HCl, pH 7.5, 1 mM EDTA
• Deoxyribonucleic acid from salmon teste, 10 mg/ml; 2 x 1 ml
• Control Yeast Plasmid DNA pRS316 carrying the ura gene; 10 μg
• Yeast Synthetic Drop-out Medium Supplement Without Uracil; 1 g

Application

Suitable for transformation of any strain of yeast. Convenient, flexible and sensitive, positive transformants can be obtained with as little as 10 ng of DNA; the optimum efficiency is in the 0.1- 3 μg range.

Features and Benefits

• Easy and ready-to-use
• Requires as little as 10 ng of plasmid DNA
• Flexibility for any strain of yeast
• Sufficient for over 100 standard transformations

General description

Sigma’s Yeast Transformation Kit contains all necessary reagents and controls for efficient transformation of yeast by the lithium acetate method.

Principle

Transformation with a plasmid complementing the mutated gene enables the transformant to grow on medium lacking the required component. Yeast cells are made competent for transformation by incubation in a buffered lithium acetate solution. Transformation is then carried out by incubating the cells together with transforming DNA and carrier DNA in a solution containing polyethylene glycol (PEG).

Price and Availability


Oxford Genetics

Kit component also available separately

Description

Product #

Add to Cart

Deoxyribonucleic acid, single stranded from salmon testes, For hybridization 2 x 1 mL MSDS D9156
Yeast Synthetic Drop-out Medium Supplements, without uracil 1 g MSDS Y1501

Kit component only

Description

Product #

Add to Cart

Transformation Buffer 100 mL MSDS    
Plate Buffer 100 mL MSDS    
pRs316 Control plasmid 10 μg MSDS    

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