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53822-U Supelco

Ascentis® Express C18, 2.7 Micron HPLC Column

2.7 μm particle size, L × I.D. 5 cm × 2.1 mm

  •  eCl@ss 32110501

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Properties

Related Categories Analytical/Chromatography, Ascentis Express C18, 2.7μm HPLC Columns, Ascentis Express Columns for Fast HPLC, C18, 2.7μm Columns, HPLC,
feature   endcapped
limit   60 °C temp. range
application(s)   HPLC: suitable
L × I.D.   5 cm × 2.1 mm
impurities   <5 ppm metals
matrix   Fused-Core particle platform
matrix active group   C18 (octadecyl) phase
particle size   2.7 μm
pore size   90 Å
operating pH   2 - 9
suitability   suitable for L1 per USP
mode of chromatography   Reversed phase

Description

Frequently Asked Questions

Live Chat and Frequently Asked Questions are available for this Product.

General description

Ascentis Express HPLC columns, through the use of Fused-Core® particle technology, can provide you with both the high speed and high efficiencies of sub-2 μm particles while maintaining lower backpressures. The combination of high efficiency and low backpressure benefits UPLC® (or other ultra high pressure system) users, as well as conventional HPLC users.
Visit the Ascentis Express home page for more information on this new column technology.

Legal Information

Ascentis is a registered trademark of Sigma-Aldrich Co. LLC

Fused-Core is a registered trademark of Advanced Materials Technology, Inc.

UPLC is a registered trademark of Waters

Price and Availability

Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles

Articles

Analysis of 25-Hydroxyvitamin D2 and 25-Hydroxyvitamin D3 | Fast LC-MS-MS

The following was generated with the assistance of an outside source using Sigma-Aldrich products. Technical content was generated and provided by:
Keywords: Absorption, Cancer, Chromatography, Clinical, Diseases, Gene expression, High performance liquid chromatography, Indicators, Liquid chromatography mass spectrometry, Mass spectrometry, Metabolites, Phase transitions, Precipitation, Sample preparations, Separation, Solid phase extractions, Vitamins

Ascentis Express UHPLC Columns with 2.0 μm Fused-Core Particles

Column Performance The new Supelco Ascentis® Express column line has been designed specifically for UHPLC instruments with 2.0 µm Fused-Core® silica, as diagrammed in Figure 1. Ascentis Express 2.0 µ...
Richard A. Henry, Gaurang Parmar
Reporter US, Volume 32.2
Keywords: High performance liquid chromatography, Mass spectrometry, Pharmaceutical, Separation

Derivatization Agents for LC/MS – An Improved Detection of Estradiol with ESI-MS

Steroid hormones are derivatives of cholesterol and play an important role in a large variety of organisms, as they can have direct control over the gene expression. 17β-estradiol (E2) controls the g...
Rudolf Köhling, Senior Scientist, LC/MS Applications
Analytix Volume 4, 2011, Article 4
Keywords: Clinical, Derivatizations, Environmental, Gene expression, High performance liquid chromatography, Hormones, Mass spectrometry, Metabolites, Pharmaceutical, Reversed-phase chromatography, Solvents

Derivatization and Separation of Aliphatic Amines

Hugh Cramer, Associate Scientist Shyam Verma, Market Segment Manager Michael Kiselewsky, Product Manager
AnalytiX, Volume 2, 2011
Keywords: Absorption, Derivatizations, High performance liquid chromatography, Separation

Fast Extraction and Low Level Detection of Chloramphenicol in Shrimp

QuEChERS Sample Preparation Using Z-Sep+ Sorbent and Analysis by Ascentis Express C18 LC-MS/MS
Emily Barrey, Olga Shimelis, Michael Halpenny, Jennifer Claus
Reporter US Volume 32.1
Keywords: Adsorption, Antibiotics, Carcinogens, Gas chromatography, Gas chromatography mass spectrometry, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Pesticides, Phase transitions, Precipitation, Sample preparations, Solid phase extractions, Solvents

Increase Bioanalytical Assay Speed via Phospholipid Removal using HybridSPE™-Precipitation Technology

One of the primary concerns when analyzing small molecules in biological samples via LC-MS/MS is ion-suppression. Ion-suppression is caused by one or more interfering components or species that co-el...
Craig Aurand, An Trinh
Reporter US Volume 26.5
Keywords: Anticoagulants, Buffers, Centrifugation, Chromatography, Coagulants, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Metabolites, Pharmaceutical, Precipitation, Reversed-phase chromatography, Size-exclusion chromatography, Solid phase extractions

Increase Bioanalytical Assay Speed via Phospholipid Removal | HybridSPE

One of the primary concerns when analyzing small molecules in biological samples via LC-MS/MS is ion-suppression. Ion-suppression is caused by one or more interfering components or species that co-el...
Craig Aurand, An Trinh
Reporter US Volume 26.5
Keywords: Anticoagulants, Buffers, Centrifugation, Chromatography, Coagulants, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Metabolites, Pharmaceutical, Precipitation, Reversed-phase chromatography, Size-exclusion chromatography, Solid phase extractions

Molecularly Imprinted Polymer SPE Increases Sensitivity for the Extraction of Clenbuterol from Urine

Clenbuterol (Figure 1) is a beta-agonist known for its growth-promoting properties in which use of the drug induces significant weight gain by increasing the proportion of muscle mass to fat. Althoug...
Olga Shimelis, Craig Aurand, An Trinh
Reporter US Volume 25.2
Keywords: Agriculture, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Protocols, Solid phase extractions

Phospholipid Extractions for Cleanup or Enrichment

Within the last 5-10 years, hydrophilic polymer SPE phases have become the phase of choice when developing new extraction methods that recover, concentrate, and cleanup small molecules (e.g., pharmac...
Carmen T. Santasania, An Trinh, and Mati Sarker
Reporter Volume 27.3
Keywords: Antibiotics, Applications, Cancer, High performance liquid chromatography, Hormones, Liquid chromatography mass spectrometry, Mass spectrometry, Metabolic Pathways, Methods, Pharmaceutical, Solid phase extractions, Solvents

Rapid Screening of Pharmaceutical Compounds | Fast HPLC

HPLC is critical to the discovery, development and eventual commercialization of pharmaceutical products. HPLC is the benchmark analytical method in the pharmaceutical industry due to its ability to ...
Hillel Brandes
Reporter EU Volume 30
Keywords: Anti-depressants, Chromatography, Diffusion, High performance liquid chromatography, Mass spectrometry, PAGE, Pharmaceutical, Reductions, Separation

Serum Lipidomics Profiling | Ascentis Express Fused-Core C18 Column

The following was generated with the assistance of an outside source using Sigma-Aldrich products. Technical content was generated and provided by:
Reporter US Volume 29.5
Keywords: Analytical Chemistry, Chromatography, Diffusion, Gas chromatography, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Metabolites, Phase transitions, Separation

Supel-Select HLB SPE for Pharmaceutical Analysis

Within the last 5-10 years, hydrophilic polymer SPE phases have become the phase of choice when developing new extraction methods that recover, concentrate, and cleanup small molecules (e.g., pharmac...
Carmen T. SantasaniaAn Trinh andMati Sarker
Reporter US Volume 27.3
Keywords: Antibiotics, Cancer, High performance liquid chromatography, Hormones, Liquid chromatography mass spectrometry, Mass spectrometry, Metabolic Pathways, Pharmaceutical, Solid phase extractions, Solvents

Related Content

Boosting HPLC Productivity: Nick the Hero [VIDEO]

Boosting HPLC Productivity Ascentis Express columns provide high-speed and high-efficiency separations that boost lab productivity. Learn about the features and benefits; become the "Nick the Hero" i...
Keywords: High performance liquid chromatography

Selection Guide for Ascentis® Express HPLC Columns

Ascentis Express C18 is the first choice for starting a new method. However, when a C18 doesn't give the desired separation or your sample contains compounds that are known to be difficult to retain ...
Keywords: High performance liquid chromatography, Separation, Supercritical fluid chromatography

Supelco HPLC Calculator for Isocratic or Gradient Method Transfer

Calculates conditions for transfer of an isocratic or gradient method from one HPLC column to another. Allows method scaling from microbore through preparative column range. Calculates time and solve...
Keywords: High performance liquid chromatography

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53501-U Ascentis® Express C18, 2.7 Micron Guard Cartridge, 2.7 μm particle size, L × I.D. 5 mm × 2.1 mm, pkg of 3 ea

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53500-U Ascentis® Express Guard Cartridge Holder, for use with Ascentis Express Guard Columns, pk of 1

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