Ascentis^® Express C18, 2.7 Micron HPLC Column

The following was generated with the assistance of an outside source using Sigma-Aldrich products. Technical content was generated and provided by:
Keywords: Absorption, Cancer, Chromatography, Clinical, Diseases, Gene expression, High performance liquid chromatography, Indicators, Liquid chromatography mass spectrometry, Mass spectrometry, Metabolites, Phase transitions, Precipitation, Sample preparations, Separation, Solid phase extractions, Vitamins

Steroid hormones are derivatives of cholesterol and play an important role in a large variety of organisms, as they can have direct control over the gene expression. 17β-estradiol (E2) controls the g...
Rudolf Köhling, Senior Scientist, LC/MS Applications
Analytix Volume 4, 2011, Article 4
Keywords: Clinical, Derivatizations, Environmental, Gene expression, High performance liquid chromatography, Hormones, Mass spectrometry, Metabolites, Pharmaceutical, Reversed-phase chromatography, Solvents

Hugh Cramer, Associate Scientist Shyam Verma, Market Segment Manager Michael Kiselewsky, Product Manager
AnalytiX, Volume 2, 2011
Keywords: Absorption, Derivatizations, High performance liquid chromatography, Separation

One of the primary concerns when analyzing small molecules in biological samples via LC-MS/MS is ion-suppression. Ion-suppression is caused by one or more interfering components or species that co-el...
Craig Aurand, An Trinh
Reporter US Volume 26.5
Keywords: Anticoagulants, Buffers, Centrifugation, Chromatography, Coagulants, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Metabolites, Pharmaceutical, Precipitation, Reversed-phase chromatography, Size-exclusion chromatography, Solid phase extractions

One of the primary concerns when analyzing small molecules in biological samples via LC-MS/MS is ion-suppression. Ion-suppression is caused by one or more interfering components or species that co-el...
Craig Aurand, An Trinh
Reporter US Volume 26.5
Keywords: Anticoagulants, Buffers, Centrifugation, Chromatography, Coagulants, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Metabolites, Pharmaceutical, Precipitation, Reversed-phase chromatography, Size-exclusion chromatography, Solid phase extractions

Clenbuterol (Figure 1) is a beta-agonist known for its growth-promoting properties in which use of the drug induces significant weight gain by increasing the proportion of muscle mass to fat. Althoug...
Olga Shimelis, Craig Aurand, An Trinh
Reporter US Volume 25.2
Keywords: Agriculture, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Solid phase extractions

Within the last 5-10 years, hydrophilic polymer SPE phases have become the phase of choice when developing new extraction methods that recover, concentrate, and cleanup small molecules (e.g., pharmac...
Carmen T. Santasania, An Trinh, and Mati Sarker
Reporter Volume 27.3
Keywords: Antibiotics, Cancer, High performance liquid chromatography, Hormones, Liquid chromatography mass spectrometry, Mass spectrometry, Metabolic Pathways, Pharmaceutical, Solid phase extractions, Solvents

HPLC is critical to the discovery, development and eventual commercialization of pharmaceutical products. HPLC is the benchmark analytical method in the pharmaceutical industry due to its ability to ...
Hillel Brandes
Reporter EU Volume 30
Keywords: Anti-depressants, Chromatography, Diffusion, High performance liquid chromatography, Mass spectrometry, PAGE, Pharmaceutical, Reductions, Separation

The following was generated with the assistance of an outside source using Sigma-Aldrich products. Technical content was generated and provided by:
Reporter US Volume 29.5
Keywords: Chromatography, Diffusion, Gas chromatography, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Metabolites, Phase transitions, Separation

Within the last 5-10 years, hydrophilic polymer SPE phases have become the phase of choice when developing new extraction methods that recover, concentrate, and cleanup small molecules (e.g., pharmac...
Carmen T. SantasaniaAn Trinh andMati Sarker
Reporter US Volume 27.3
Keywords: Antibiotics, Cancer, High performance liquid chromatography, Hormones, Liquid chromatography mass spectrometry, Mass spectrometry, Metabolic Pathways, Pharmaceutical, Solid phase extractions, Solvents

Ascentis Express C18 is the first choice for starting a new method. However, when a C18 doesn't give the desired separation or your sample contains compounds that are known to be difficult to retain ...
Keywords: High performance liquid chromatography, Separation, Supercritical fluid chromatography

Calculates conditions for transfer of an isocratic or gradient method from one HPLC column to another. Allows method scaling from microbore through preparative column range. Calculates time and solve...
Keywords: High performance liquid chromatography