|Related Categories||3 micron, Analytical/Chromatography, Ascentis C8, Ascentis HPLC Columns, HPLC,|
|extent of labeling||15% Carbon loading|
|limit||≤70 °C temp. range|
|L × I.D.||10 cm × 1.0 mm|
|surface coverage||4.0 μmol/m2|
|impurities||<5 ppm metals|
|matrix||silica gel high purity, spherical|
|matrix active group||octylsilane phase|
|particle size||3 μm|
|pore size||100 Å|
|surface area||450 m2/g|
|operating pH range||2 - 8|
|suitability||suitable for L7 per USP|
• Superior retention for hydrophobic molecules
• Excellent peak shape
• Low bleed LC-MS separations
The Ascentis family of columns is the fourth generation of HPLC column technology from Supelco scientists. Ascentis columns are bonded on high purity, 100 Angstrom silica including 3, 5, and 10 micron particle size. Columns are designed for small molecule applications and are scalable from micro columns (1.0 mm I.D.) to preparative dimensions (50 mm I.D.). The family includes C18, C8, Phenyl, Si and embedded polar group phase, RP-Amide.
The Ascentis C8 is a highly reproducible column with excellent selectivity towards polar compounds. The phase excells in highly aqueous mobile phases as well as high organic mobile phases.
Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
Isoprenoid pathway metabolites such isoprenoidphosphates and-pyrophosphates are central biological intermediates leading to sterols, dolichols, ubiquinones, prenylated natural products and proteins. ...
Rudolf Köhling, Roland Meier, Bernhard Schönenberger and Roland Wohlgemuth
Sigma-Aldrich®, Buchs, Switzerland
Biofiles, Vol. 8, No. 17
Calculates conditions for transfer of an isocratic or gradient method from one HPLC column to another. Allows method scaling from microbore through preparative column range. Calculates time and solve...
Keywords: High performance liquid chromatography
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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