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  • 130672 - N-Hydroxysuccinimide


130672 Aldrich



Synonym: 1-Hydroxy-2,5-pyrrolidinedione, HOSu, NHS



Frequently Asked Questions

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Additive used in the carbodiimide method for improved amidations1 and peptide couplings.2

Used to activate a carboxyl group for amide bond formation.


1 kg in poly bottle

5 kg in poly drum

5, 25, 100, 250 g in poly bottle

Price and Availability

Suggested Laboratory Gloves

Laboratory GlovesThis substance has been tested against several types of hand protection for CE compliance. Click below to find the recommended gloves for handling this product.

Safety & Documentation

Safety Information

WGK Germany 


Certificate of Analysis

Certificate of Origin

Protocols & Articles


Molecular Monolayers on Silicon Surfaces

Controlled formation of organic molecular monolayers on silicon surfaces offers the promise of enhancing the functionality of existing and emerging silicon based materials and devices. The synthetic ...
Gregory P. Lopinski, Daniel D.M. Wayner
Material Matters 2008, 3.2, 38.
Keywords: Adsorption, Applications, Buffers, Degradations, Diffusion, Electronics, Events, Gas chromatography, Grignard Reaction, Hydrosilylations, Immobilization, Infrared spectroscopy, Methods, Microelectronics, Microscopy, Nanomaterials, Nucleic acid hybridization, Oxidations, Reductions, Semiconductor, Solvents, Sonication, Spectra, Surface Science, Transduction

Peer-Reviewed Papers


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1. A method for the high efficiency of water-soluble carbodiimide-mediated amidation. Anal. Biochem. 218, 87, (1994)


2. Polycyclic Aromat. Compd. 3, 781, (1993)

Multimodal image-guided enzyme/prodrug cancer therapy. J. Am. Chem. Soc. 128, 15072, (2006)


Structure activity relationship studies on the antimicrobial activity of novel edeine A and D analogues. J. Pept. Sci. 12, 653, (2006)


Copper-free click biofunctionalization of silicon nitride surfaces via strain-promoted alkyne-azide cycloaddition reactions. Manova RK, Pujari SP, Weijers CA, et al. Langmuir 28(23), 8651-63, (2012)


Rubber elasticity for incomplete polymer networks. Nishi K, Chijiishi M, Katsumoto Y, et al. J. Chem. Phys. 137(22), 224903, (2012)


Surface modifications of microprojection arrays for improved biomarker capture in the skin of live mice. Bhargav A, Muller DA, Kendall MA, et al. ACS Appl. Mater. Interfaces 4(5), 2483-9, (2012)


Fibroblast-derived matrix (FDM) as a novel vascular endothelial growth factor delivery platform. Du P, Hwang MP, Noh YK, et al. J. Control. Release 194, 122-9, (2014)

Noxious compounds activate TRPA1 ion channels through covalent modification of cysteines. Macpherson, L.J., et al. Nature, 1-4, (2007)


Immobilization of hydrophobic peptidic ligands to hydrophilic chromatographic matrix: a preconcentration approach. Gautam S and Loh KC Anal. Biochem. 423(2), 202-9, (2012)


Malachite green derivative-functionalized single nanochannel: light-and-pH dual-driven ionic gating. Wen L, Liu Q, Ma J, et al. Adv. Mater. 24(46), 6193-8, (2012)


Preparation of hydrophilic carbon-functionalized magnetic microspheres coated with chitosan and application in solid-phase extraction of bisphenol A in aqueous samples. Geng Y, Ding M, Chen H, et al. Talanta 89, 189-94, (2012)


Hyaluronic acid-human blood hydrogels for stem cell transplantation. Chang CY, Chan AT, Armstrong PA, et al. Biomaterials 33(32), 8026-33, (2012)


Synthesis of multifunctional Ag@Au@phenol formaldehyde resin particles loaded with folic acids for photothermal therapy. Yang P, Xu QZ, Jin SY, et al. Chemistry 18(30), 9294-9, (2012)


Quantitative methods based on twisted nematic liquid crystals for mapping surfaces patterned with bio/chemical functionality relevant to bioanalytical assays. Lowe AM Anal. Chem. 80(8), 2637-45, (2008)


Gas-phase conjugation to arginine residues in polypeptide ions via N-hydroxysuccinimide ester-based reagent ions. McGee WM, Mentinova M, and McLuckey SA J. Am. Chem. Soc. 134(28), 11412-4, (2012)


Rapid quantification of histamine in human psoriatic plaques using microdialysis and ultra high performance liquid chromatography with fluorescence detection. Guihen E, Ho WL, Hogan AM, et al. J. Chromatogr. B. Analyt. Technol. Biomed. Life Sci. 880(1), 119-24, (2012)


Optimizing the enrichment of cross-linked products for mass spectrometric protein analysis. Fritzsche R, Ihling CH, Götze M, et al. Rapid Commun. Mass Spectrom. 26(6), 653-8, (2012)


Effect of fish collagen modification on its thermal and rheological properties. Safandowska M and Pietrucha K Int. J. Biol. Macromol. 53, 32-7, (2013)


Highly efficient click labeling using 2-[¹⁸F]fluoroethyl azide and synthesis of an ¹⁸FN-hydroxysuccinimide ester as conjugation agent. Zhou D, Chu W, Dence CS, et al. Nucl. Med. Biol. 39(8), 1175-81, (2012)


Ring-opening metathesis polymerization-based recyclable magnetic acylation reagents. Kainz QM, Linhardt R, Maity PK, et al. ChemSusChem 6(4), 721-9, (2013)


Aerobic amide bond formation with N-hydroxysuccinimide. Yao H and Yamamoto K Chem. Asian J. 7(7), 1542-5, (2012)


Surface functionalized nanofibrillar cellulose (NFC) film as a platform for immunoassays and diagnostics. Orelma H, Filpponen I, Johansson LS, et al. Biointerphases 7(1-4), 61, (2012)


High-resolution single-molecule recognition imaging of the molecular details of ricin-aptamer interaction. Wang B, Guo C, Zhang M, et al. J. Phys. Chem. B 116(17), 5316-22, (2012)


Development of D-lysine-assisted 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide/N-hydroxysuccinimide-initiated cross linking of collagen matrix for design of scaffold. Krishnamoorthy G, Sehgal PK, Mandal AB, et al. J. Biomed. Mater. Res. A 101(4), 1173-83, (2013)


Dimerization of an immunoactivating peptide derived from mycobacterial hsp65 using N-hydroxysuccinimide based bifunctional reagents is critical for its antitumor properties. Bezouška K, Kubínková Z, Stříbný J, et al. Bioconjug. Chem. 23(10), 2032-41, (2012)


Palmitate-derivatized human IL-2: a potential anticancer immunotherapeutic of low systemic toxicity. Chou SH, Shetty AV, Geng Y, et al. Cancer Immunol. Immunother. 62(3), 597-603, (2013)


Structural identification and biological activity of positional isomers of long-acting and mono-PEGylated recombinant human granulocyte colony-stimulating factor with trimeric-structured methoxy polyethylene glycol N-hydroxysuccinimidyl functional group. Son JP, Jun SW, Choi YK, et al. Anal. Biochem. 423(2), 286-93, (2012)


Surface-bound basement membrane components accelerate amyloid-β peptide nucleation in air-free wells: an in vitro model of cerebral amyloid angiopathy. Hasegawa K, Ozawa D, Ookoshi T, et al. Biochim. Biophys. Acta 1834(8), 1624-31, (2013)


Using small molecule reagents to selectively modify epitopes based on their conformation. Silva CJ Prion 6(2), 163-73, (2012)


Monodispersed molecularly imprinted polymer for creatinine by modified precipitation polymerization. Haginaka J, Miura C, Funaya N, et al. Anal. Sci. 28(4), 315-7, (2012)


A monoclonal antibody specific for Δ12-prostaglandin J2 and its utilization in the immunological assay in cell culture system of adipocytes. Syeda PK, Hossain MS, Chowdhury AA, et al. Hybridoma (Larchmt.) 31(5), 364-71, (2012)


Chemical cross-linking and high-resolution mass spectrometry to study protein-drug interactions. Müller MQ and Sinz A Methods Mol. Biol. 803, 205-18, (2012)


Merck 14,10330

Beil. 21,V,9,498

Fieser 1,487 / Fieser 9,246

Aldrich MSDS 1, 1080:A / Corp MSDS 1 (1), 1962:B / FT-IR 1 (1), 796:B / FT-IR 2 (1), 1351:A / FT-NMR 1 (1), 1300:B / IR-Spectra (2), 418:G / IR-Spectra (3), 472:B / NMR-Reference 2 (1), 667:C / RegBook 1 (1), 931:I / Sigma FT-IR 1 (2), 646:B / Structure Index 1, 145:A:2

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