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165344 Aldrich

1-[3-(Dimethylamino)propyl]-3-ethylcarbodiimide methiodide

Synonym: EDC methiodide



Related Categories Carbodiimides, Chemical Biology, Chemical Synthesis, Coupling, Peptide Chemistry,
mp   97-99 °C(lit.)
storage temp.   2-8°C



1, 10 g in glass bottle


• Crosslinking agent1

Reactant for:
• Amide bond forming (amidation) crosslinking reactions2,3

Water-soluble carboxyl modifying reagent for proteins.

Price and Availability

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1-<WBR>(3-<WBR>Dimethylaminopropyl)<WBR>-<WBR>3-<WBR>ethylcarbodiimide, polymer-bound

200-400 mesh, 2 % cross-linked with divinylbenzene

Collagen human

Bornstein and Traub Type I, recombinant, expressed in Nicotiana


1.0 M in methylene chloride

Safety & Documentation

Safety Information

GHS07  GHS07
Signal word 
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
WGK Germany 
Protocols & Articles



Carbodiimide-mediated peptide coupling remains to the most frequently used technique. As a major advantage, carbodiimides do not require prior activation of the carboxylic acid. Dicyclohexylcarbodiim...
Matthias Junkers
Aldrich ChemFiles 2007, 7.2, 5.
Keywords: Cyclizations, Peptide synthesis, Solid phase peptide synthesis

Peer-Reviewed Papers


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1. Orthogonally dual-clickable Janus nanoparticles via a cyclic templating strategy. Zhang, S.; et al. J. Am. Chem. Soc. 133, 11046, (2011)


2. Song, S.-J.; et al. J. Mater. Chem. 19, 3248, (2009)

3. Turner, J. L.; et al. Adv. Funct. Mater. 15, 1248, (2005)

Interaction of cytochrome c with cytochrome c oxidase studied by monoclonal antibodies and a protein modifying reagent. Biochemistry 31, 9090, (1992)


Structural and functional analysis of the coupling subunit F in solution and topological arrangement of the stalk domains of the methanogenic A1AO ATP synthase. Schäfer I, Rössle M, Biuković G, et al. J. Bioenerg. Biomembr. 38(2), 83-92, (2006)


[Investigation of functional groups of Cryptococcus albidus alpha-L-rhamnosidase]. Gudzenko OV and Varbanets' LD Mikrobiol. Z. 74(4), 19-28, (2012)


[Synthesis and identification of penicillic-acid antigens from Penicillium cyclopium]. Lei H, Yuan H, Wu J, et al. Sheng Wu Gong Cheng Xue Bao 24(5), 898-902, (2008)


[Chemical modification of heparin]. Ponedel'kina IIu, Odinokova VN, Lukina ES, et al. Bioorg. Khim. 32(5), 524-9, (2006)


[Conjugation and immunogenic evaluation of complete immunogen for the small molecular environmental pollutants 2,4-D]. Yu RZ, He M, Shi HC, et al. Huan Jing Ke Xue 27(1), 146-50, (2006)


Characterization of porous collagen/hyaluronic acid scaffold modified by 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide cross-linking. Park SN, Park JC, Kim HO, et al. Biomaterials 23(4), 1205-12, (2002)


Interfacing living yeast cells with graphene oxide nanosheaths. Yang SH, Lee T, Seo E, et al. Macromol. Biosci. 12(1), 61-6, (2012)


Wound closure with EDC cross-linked cultured skin substitutes grafted to athymic mice. Powell HM and Boyce ST Biomaterials 28(6), 1084-92, (2007)


Subunit interactions in the clathrin-coated vesicle vacuolar (H(+))-ATPase complex. Xu T, Vasilyeva E, and Forgac M J. Biol. Chem. 274(41), 28909-15, (1999)


Structural changes in the actin-myosin cross-bridges associated with force generation induced by temperature jump in permeabilized frog muscle fibers. Tsaturyan AK, Bershitsky SY, Burns R, et al. Biophys. J. 77(1), 354-72, (1999)


Strain variation in autoimmunity: attempted tolerization of DA rats results in the induction of experimental autoimmune encephalomyelitis. Lenz DC, Wolf NA, and Swanborg RH J. Immunol. 163(4), 1763-8, (1999)


Synthesis of poly(Pro-Hyp-Gly)(n) by direct poly-condensation of (Pro-Hyp-Gly)(n), where n=1, 5, and 10, and stability of the triple-helical structure. Kishimoto T, Morihara Y, Osanai M, et al. Biopolymers 79(3), 163-72, (2005)


Subunit proximity in the H+-translocating NADH-quinone oxidoreductase probed by zero-length cross-linking. Kao MC, Matsuno-Yagi A, and Yagi T Biochemistry 43(12), 3750-5, (2004)


Functional dissection of trigger factor and DnaK: interactions with nascent polypeptides and thermally denatured proteins. Schaffitzel E, Rüdiger S, Bukau B, et al. Biol. Chem. 382(8), 1235-43, (2001)


Rotations of a few cross-bridges in muscle by confocal total internal reflection microscopy. Borejdo J, Talent J, Akopova I, et al. Biochim. Biophys. Acta 1763(2), 137-40, (2006)


States of thin filament regulatory proteins as revealed by combined cross-linking/X-ray diffraction techniques. Iwamoto H, Oiwa K, Suzuki T, et al. J. Mol. Biol. 317(5), 707-20, (2002)


The effect of amino acid-modifying reagents on chloroplast protein import and the formation of early import intermediates. Row PE and Gray JC J. Exp. Bot. 52(354), 57-66, (2001)


The location of the mobile electron carrier ferredoxin in vascular plant photosystem I. Ruffle SV, Mustafa AO, Kitmitto A, et al. J. Biol. Chem. 275(46), 36250-5, (2000)


Quantification of residual EDU (N-ethyl-N'-(dimethylaminopropyl) carbodiimide (EDC) hydrolyzed urea derivative) and other residual by LC-MS/MS. Lei QP, Lamb DH, Shannon AG, et al. J. Chromatogr. B. Analyt. Technol. Biomed. Life Sci. 813(1-2), 103-12, (2004)


Hydrodynamic properties and quaternary structure of the 90 kDa heat-shock protein: effects of divalent cations. Garnier C, Barbier P, Devred F, et al. Biochemistry 41(39), 11770-8, (2002)


Horseradish peroxidase immobilized through its carboxylic groups onto a polyacrylonitrile membrane: comparison of enzyme performances with inorganic beaded supports. Leirião PR, Fonseca LJ, Taipa MA, et al. Appl. Biochem. Biotechnol. 110(1), 1-10, (2003)


New cationized LDL-DNA complexes: their targeted delivery to fibroblasts in culture. Khan Z, Hawtrey AO, and Ariatti M Drug Deliv. 10(3), 213-20, (2003)


Identification of functionally important acidic residues in transducin by group-specific labeling. Kosoy A, Möller C, Perdomo D, et al. Biol. Res. 36(3-4), 389-404, (2003)


Enzymatic properties of sialic acid binding lectin from Rana catesbeiana modified with a water-soluble carbodiimide in the presence of various nucleophiles. Iwama M, Ogawa Y, Ohgi K, et al. Biol. Pharm. Bull. 24(12), 1366-9, (2001)


Influence of paraformaldehyde and EDAC fixation on the demonstrability of hormones (histamine, endorphin, triiodothyronine) in rat immune cells: an immunocytochemical comparative analysis. Csaba G, Kovács P, and Pállinger E Cell Biol. Int. 30(5), 412-5, (2006)


Merck 14,10328

Corp MSDS 1 (1), 1344:B / FT-NMR 1 (1), 1413:C / RegBook 1 (1), 1035:E / Structure Index 1, 160:D:8

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