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  • 375934 - N-tert-Butyldimethylsilyl-N-methyltrifluoroacetamide with 1% tert-Butyldimethylchlorosilane


375934 Aldrich

N-tert-Butyldimethylsilyl-N-methyltrifluoroacetamide with 1% tert-Butyldimethylchlorosilane


Synonym: N-Methyl-N-tert-butyldimethylsilyltrifluoroacetamide, MTBSTFA + 1% TBDMSCl, MTBSTFA+TBDMSCl, 99:1, Silylating mixture Fluka VI

  • CAS Number 77377-52-7

  • Empirical Formula (Hill Notation) C9H18F3NOSi

  • Molecular Weight 241.33

  •  Beilstein Registry Number 3606546

  •  MDL number MFCD00009671

  •  PubChem Substance ID 24863412

  • Popular Documents:  FTIR (PDF)  




Preparation of N-tert-butyldimethylsilyl ethanolamines resulting from hydrolysis of nitrogen mustards.

Selective O-silylation of N,O-diacylhydroxylamines.1


10×1 mL in ampule

5, 25 mL in glass bottle

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pkg of 20 × 1 mL

for GC derivatization


anhydrous, 99.8%

Safety & Documentation

Safety Information

Signal word 
Hazard statements 
Precautionary statements 
UN 1993C 3 / PGIII
WGK Germany 
Flash Point(F) 
113 °F
Flash Point(C) 
45 °C

Protocols & Articles


Fluka Silylating Reagents for GC-MS Compatible Derivatives of NSAIDs

Human and veterinary pharmaceuticals are a group of “emerging” contaminants,1, 2 some of which are produced in increasingly large volumes every year. The amounts produced are reaching quantities simi...
Luis-Alberto Martin
AnalytiX Volume 10 Article 2
Keywords: Anti-inflammatory agents, Derivatizations, Environmental, Gas chromatography, Gas chromatography mass spectrometry, Mass spectrometry, Metabolites, Pesticides, Pharmaceutical, Silylations, Veterinary

Peer-Reviewed Papers


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1. Organometallics 23, 2157-2161, (2004)

Determination of nitrogen mustard hydrolysis products, ethanolamines by gas chromatography-mass spectrometry after tert-butyldimethylsilyl derivatization. J. Chromatogr. A. 1122, 242, (2006)


Comparison of MTBSTFA and BSTFA in derivatization reactions of polar compounds prior to GC/MS analysis. Schummer C, Delhomme O, Appenzeller BM, et al. Talanta 77(4), 1473-82, (2009)


Solid phase microextraction procedure for the determination of alkylphenols in water by on-fiber derivatization with N-tert-butyl-dimethylsilyl-N-methyltrifluoroacetamide. Pan YP and Tsai SW Anal. Chim. Acta 624(2), 247-52, (2008)


Determination of ethephon residues in water by gas chromatography with cubic mass spectrometry after ion-exchange purification and derivatisation with N-(tert-butyldimethylsilyl)-N-methyltrifluoroacetamide. Royer A, Laporte F, Bouchonnet S, et al. J. Chromatogr. A. 1108(1), 129-35, (2006)


Simultaneous analysis of pesticides from different chemical classes by using a derivatisation step and gas chromatography-mass spectrometry. Raeppel C, Nief M, Fabritius M, et al. J. Chromatogr. A. 1218(44), 8123-9, (2011)


Large-scale 13C-flux analysis reveals distinct transcriptional control of respiratory and fermentative metabolism in Escherichia coli. Haverkorn van Rijsewijk BR, Nanchen A, Nallet S, et al. Mol. Syst. Biol. 7, 477, (2011)


Optimization of silylation using N-methyl-N-(trimethylsilyl)-trifluoroacetamide, N,O-bis-(trimethylsilyl)-trifluoroacetamide and N-(tert-butyldimethylsilyl)-N-methyltrifluoroacetamide for the determination of the estrogens estrone and 17alpha-ethinylestradiol by gas chromatography-mass spectrometry. J. Chromatogr. A. 1108, 121, (2006)


Dispersive liquid-liquid microextraction applied to the simultaneous derivatization and concentration of triclosan and methyltriclosan in water samples. Montes R, Rodríguez I, Rubí E, et al. J. Chromatogr. A. 1216(2), 205-10, (2009)


Optimization of solid-phase microextraction conditions for the determination of triclosan and possible related compounds in water samples. Canosa P, Rodriguez I, Rubí E, et al. J. Chromatogr. A. 1072(1), 107-15, (2005)


Differential metabolomics using stable isotope labeling and two-dimensional gas chromatography with time-of-flight mass spectrometry. Huang X and Regnier FE Anal. Chem. 80(1), 107-14, (2008)


Analysis of endocrine disrupting compounds, pharmaceuticals and personal care products in sewage sludge by gas chromatography-mass spectrometry. Yu Y and Wu L Talanta 89, 258-63, (2012)


One-step extraction and derivatization liquid-phase microextraction for the determination of chlorophenols by gas chromatography-mass spectrometry. Wang X, Luo L, Ouyang G, et al. J. Chromatogr. A. 1216(35), 6267-73, (2009)


Determination of hydroxylated metabolites of polycyclic aromatic hydrocarbons in sediment samples by combining subcritical water extraction and dispersive liquid-liquid microextraction with derivatization. Wang X, Lin L, Luan T, et al. Anal. Chim. Acta 753, 57-63, (2012)


Determination of 14 benzodiazepines and hydroxy metabolites, zaleplon and zolpidem as tert-butyldimethylsilyl derivatives compared with other common silylating reagents in whole blood by gas chromatography-mass spectrometry. Gunnar T, Ariniemi K, and Lillsunde P J. Chromatogr. B. Analyt. Technol. Biomed. Life Sci. 818(2), 175-89, (2005)


Optimization of N-methyl-N-[tert-butyldimethylsilyl]trifluoroacetamide as a derivatization agent for determining isotopic enrichment of glycerol in very-low density lipoproteins. Adiels M, Larsson T, Sutton P, et al. Rapid Commun. Mass Spectrom. 24(5), 586-92, (2010)


A multiresidue method for the analysis of phenols and nitrophenols in the atmosphere. Morville S, Scheyer A, Mirabel P, et al. J. Environ. Monit. 6(12), 963-6, (2004)


Neurochemical analysis of amino acids, polyamines and carboxylic acids: GC-MS quantitation of tBDMS derivatives using ammonia positive chemical ionization. Wood PL, Khan MA, and Moskal JR J. Chromatogr. B. Analyt. Technol. Biomed. Life Sci. 831(1-2), 313-9, (2006)


The detection of various opiates and benzodiazepines by comprehensive two-dimensional gas chromatography/time-of-flight mass spectrometry. Guthery B, Bassindale A, Pillinger CT, et al. Rapid Commun. Mass Spectrom. 23(3), 340-8, (2009)


Excretion of 2,3-dihydroxy-propionamide (OH-PA), the hydrolysis product of glycidamide, in human urine after single oral dose of deuterium-labeled acrylamide. Hartmann EC, Latzin JM, Schindler BK, et al. Arch. Toxicol. 85(6), 601-6, (2011)


Simultaneous retention index analysis of urinary amino acids and carboxylic acids for graphic recognition of abnormal state. Paik MJ, Lee HJ, and Kim KR J. Chromatogr. B. Analyt. Technol. Biomed. Life Sci. 821(1), 94-104, (2005)


Artifacts related to N-methyl-N-(tert-butyldimethylsilyl)trifluoroacetamide derivatization of citrulline revealed by gas chromatography-mass spectrometry using both electron and chemical ionization. Smith PA, Villa V, and King GL J. Chromatogr. A. 1217(33), 5444-8, (2010)


Selection of the derivatization reagent--the case of human blood cholesterol, its precursors and phytosterols GC-MS analyses. Saraiva D, Semedo R, Castilho Mda C, et al. J. Chromatogr. B. Analyt. Technol. Biomed. Life Sci. 879(32), 3806-11, (2011)


Simultaneous determination of 2-naphthol and 1-hydroxy pyrene in urine by gas chromatography-mass spectrometry. Shin HS and Lim HH J. Chromatogr. B. Analyt. Technol. Biomed. Life Sci. 879(7-8), 489-94, (2011)


Comparison and evaluation of analysis procedures for the quantification of (2-methoxyethoxy)acetic acid in urine. B'Hymer C, Butler MA, and Cheever KL Anal. Bioanal. Chem 383(2), 201-9, (2005)


Simultaneous analysis of lysine, Nepsilon-carboxymethyllysine and lysinoalanine from proteins. Bosch L, Sanz ML, Montilla A, et al. J. Chromatogr. B. Analyt. Technol. Biomed. Life Sci. 860(1), 69-77, (2007)


Determination of creatine and guanidinoacetate by GC-MS: study of their stability in urine at different temperatures. Prieto JA, Andrade F, Martín S, et al. Clin. Biochem. 42(1-2), 125-8, (2009)


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