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457779 Aldrich

DL-Dithiothreitol

99%

Synonym: threo-1,4-Dimercapto-2,3-butanediol, Cleland’s reagent, DTT

  • CAS Number 3483-12-3

  • Linear Formula HSCH2CH(OH)CH(OH)CH2SH

  • Molecular Weight 154.25

  •  Beilstein Registry Number 1719757

  •  EC Number 222-468-7

  •  MDL number MFCD00004877

  • Popular Documents:  FTNMR (PDF)  

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Properties

InChI Key   VHJLVAABSRFDPM-QWWZWVQMSA-N
assay   99%
mp   41-44 °C(lit.)

Description

Application

An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).

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Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
RIDADR 
UN 3335
WGK Germany 
3
RTECS 
EK1610000
Flash Point(F) 
235.4 °F
Flash Point(C) 
113 °C
Protocols & Articles
Peer-Reviewed Papers
15

References

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D0632 DL-Dithiothreitol, ≥98% (HPLC), ≥99.0% (titration)

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