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608041 Aldrich

L-Lysine-13C6,15N2 hydrochloride

99 atom % 13C, 99 atom % 15N, 95% (CP)

Synonym: (S)-2,6-Diaminohexanoic acid-13C6,15N2 hydrochloride, 13C and 15N Labeled Lys, 13C and 15N Labeled lysine hydrochloride, SILAC Amino Acid

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Properties

Related Categories Alphabetical Listings, Amino Acids & Derivatives, Amino Acids and Derivatives, Biomolecular NMR, I-L,
InChI Key   BVHLGVCQOALMSV-GSMNGUNQSA-N
isotopic purity   99 atom % 13C
  99 atom % 15N
assay   95% (CP)
optical activity   [α]25/D +20.7°, c = 2 in 5 M HCl
mp   263-264 °C (dec.)(lit.)
mass shift   M+8

Description

Packaging

1, 2 g in glass bottle

100, 250 mg in glass bottle

This product may be available from bulk stock and can be packaged on demand. For information on pricing, availability and packaging, please contact Stable Isotopes Customer Service.

Application

• L-Lysine-13C6,15N2 hydrochloride has been used in pSILAC (pulsed stable isotope labeling by amino acids in cell culture) mass spectrometry to study protein profile in cells transfected with microRNAs.
• It has been used in SILAC isotopic labeling for the characterization of lysine racemase.
• It has been used in SILAC labeling for phosphoproteomic measurements in neuro-2a cells treated with and without GFKP-19 (2-pyrrolidone derivative).

General description

Lysine is a basic ketogenic amino acid with a protonated alkyl amino group. Lysine degradation results in the formation of acetoacetate. Acetylation/deacetylation of lysine residues in histones is a mechanism to regulate chromatin organization in eukaryotes. Isotope labeled amino acids are required for the production of isotopically labeled proteins.

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles
Peer-Reviewed Papers
15

References

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D9443 Dulbecco’s Modified Eagle’s Medium - low glucose, liquid, sterile-filtered, suitable for cell culture, designed for isotope labeling in cell culture applications
R1780 RPMI-1640 Medium, With L-glutamine and sodium bicarbonate. Without arginine, leucine, lysine, and phenol red, liquid, sterile-filtered, suitable for cell culture, designed for isotope labeling for cell culture applications
749907 L-Lysine-13C6,15N2,2,3,3,4,4,5,5,6,6-d9 monohydrochloride, 98 atom % D, 99 atom % 13C, 98 atom % 15N, 95 atom % (CP)

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