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806013 Aldrich

APN-BCN

97%

Synonym: Bicyclo[6.1.0]non-4-yn-9-ylmethyl (4-(cyanoethynyl)phenyl)carbamate

  • Empirical Formula (Hill Notation) C20H18N2O2

  • Molecular Weight 318.37

  •  PubChem Substance ID 329768983

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Properties

Related Categories Chemical Biology, Chemical Synthesis, Crosslinkers, Linkers and Crosslinkers, Other Reactivities More...
InChI Key   LVFLVSUFJDGHRN-UHFFFAOYSA-N
assay   97%
form   powder
storage temp.   −20°C

Description

Packaging

50 mg in glass bottle

Preparation Note

Standard protein labeling procedure (cysteine labeling).

• Dissolve the protein in the appropriate buffer* with pH 6.5-9.0 (e.g. PBS) at 1-10 mg/mL concentration.
• Apply the appropriate amount of the stock solution of the reagent (1-5 molar eq. per free cysteine residue).
• Incubate at room temperature for 2 hours.
• If necessary; purify the protein-BCN conjugate using size exclusion chromatography or ultrafiltration.
• The conjugate can be readily coupled with azide-containing molecules by incubating the components in an aqueous buffer.
*Note: avoid thiol-containing buffers.

Application

APN-BCN is a bifunctional crosslinker for thiol-to-azide coupling. The coupling can be performed with high selectivity in biological medium using mild reaction conditions.

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Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers
15

References

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