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806021 Aldrich

APN-CHO

Synonym: 3-(4-formylphenyl)propiolonitrile, APN-aldehyde

  • Empirical Formula (Hill Notation) C10H5NO

  • Molecular Weight 155.15

  •  PubChem Substance ID 329768984

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Properties

Related Categories Amine to Sulfhydryl, Chemical Biology, Chemical Synthesis, Crosslinkers, Linkers and Crosslinkers More...
InChI Key   LYISHMGQKXLBHI-UHFFFAOYSA-N
form   powder
storage temp.   2-8°C

Description

Packaging

50 mg in glass bottle

Preparation Note

Standard protein labeling procedure (cysteine labeling).

• Dissolve the protein in the appropriate buffer with pH 6.5-9.0 (e.g. PBS) at 1-10 mg/mL concentration.
• Apply the appropriate amount of the stock solution of the reagent (1-5 molar eq. per free cysteine residue).
• Incubate at room temperature for 2 hours.
• If necessary, purify the protein-aldehyde conjugate using size exclusion chromatography or ultrafiltration.
• The conjugate can be readily coupled with hydrazides/hydrazones/hydroxylamines using standard protocols.

Application

APN-Aldehyde is a bifunctional crosslinker for introduction of aldehyde into biomolecules. The coupling can be performed with high selectivity in biological medium using mild reaction conditions.

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers
15

References

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