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858811 Aldrich

5-Bromo-2′-deoxyuridine

97%

Synonym: 5-BrdU, 5-Bromo-1-(2-deoxy-β-D-ribofuranosyl)uracil, 5-Bromouracil deoxyriboside, BUdR

  • CAS Number 59-14-3

  • Empirical Formula (Hill Notation) C9H11BrN2O5

  • Molecular Weight 307.10

  •  Beilstein Registry Number 30395

  •  EC Number 200-415-9

  •  MDL number MFCD00006529

  •  PubChem Substance ID 24888474

  • Popular Documents:  FTNMR (PDF)  

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Properties

InChI Key   WOVKYSAHUYNSMH-RRKCRQDMSA-N
assay   97%
optical activity   [α]22/D +23.0°, c = 1 in H2O
mp   191-194 °C (dec.)(lit.)
storage temp.   −20°C

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Biochem/physiol Actions

Thymidine analog used as a mutagen in genetic research. Selectively incorporated into cellular DNA during S-phase.

Price and Availability

Safety & Documentation

Safety Information

Symbol 
GHS08  GHS08
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
2
RTECS 
YU7350000

Documents

Certificate of Analysis

Certificate of Origin

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the stability of Product 858811, 5-Bromo-2′-deoxyuridine (BrdU)?
The comparable Sigma product, B5002, has a recommended retest specification of 2 years. We have observed by TLC that the material is unchanged after 3 years. Solutions at neutral pH should be stable if stored frozen for up to 6 months.
What can I use to detect BrdU, 5-Bromo-2′-deoxyuridine, in vivo and in vitro?
For the immunohistochemical detection of BrdU, we recommend the monoclonal anti-BrdU antibody, Product No. B2531.
What are the hazards associated with Product 858811, 5-Bromo-2′-deoxyuridine (BrdU)?
BrdU is a known carcinogen, and caution should be exercised when handling. Please refer to the MSDS by clicking on MSDS link from the product for 858811: http://www.sigmaaldrich.com/catalog/search/ProductDetail/ALDRICH/858811
How long do I treat cells with Product 858811, 5-Bromo-2′-deoxyuridine (BrdU) to incorporate it into the DNA?
Actively growing cells may be treated with 10 µM BrdU for 1 hour.
How does Product 858811, 5-Bromo-2′-deoxyuridine (BrdU), work as a thymidine analog?
BrdU replaces thymidine during the S-phase of cell division. To view this information in the Sigma B5002 product information sheet, click on the following link: http://www.sigmaaldrich.com/sigma/product%20information%20sheet/B5002pis.pdf
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
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Protocols & Articles
Peer-Reviewed Papers
15

References

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B5002 5-Bromo-2′-deoxyuridine, ≥99% (HPLC)

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