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860336 Aldrich

3,3′,5,5′-Tetramethylbenzidine

≥99%

DOWNLOAD MSDS (PDF)

Synonym: BM blue Sure Blue TMB TMB Blotting Plus TMB substrate TMB

  • CAS Number 54827-17-7

  • Linear Formula [-C6H2(CH3)2-4-NH2]2

  • Molecular Weight 240.34

  •  Beilstein Registry Number 2808541

  •  EC Number 259-364-6

  •  MDL number MFCD00007748

  •  PubChem Substance ID 24888505

3,3′,5,5′-Tetramethylbenzidine ≥99%
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Properties

Related Categories Amines, Building Blocks, C11 to C38, Chemical Synthesis, Nitrogen Compounds,
assay   ≥99%
mp   168-171 °C(lit.)

Description

Application

3,3′,5,5′-Tetramethylbenzidine (TMB) is a noncarcinogenic substitute (Ames test negative) for benzidine1 suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm. A sensitive and specific reagent for the detection of blood,2 assay of hemoglobin,3,4,5 assay of peroxidases.6,7,8

Packaging

1, 5, 25 g in glass btl

100 mg in glass btl

Price and Availability

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