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860336 Aldrich

3,3′,5,5′-Tetramethylbenzidine

≥99%

Synonym: BM blue, Sure Blue TMB, TMB Blotting Plus, TMB substrate, TMB

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Properties

Related Categories Amines, Building Blocks, C15 to C19, Chemical Synthesis, Nitrogen Compounds,
InChI Key   UAIUNKRWKOVEES-UHFFFAOYSA-N
assay   ≥99%
mp   168-171 °C(lit.)
storage temp.   2-8°C

Description

Application

3,3′,5,5′-Tetramethylbenzidine (TMB) is a noncarcinogenic substitute (Ames test negative) for benzidine suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm. A sensitive and specific reagent for the detection of blood, assay of hemoglobin, assay of peroxidases.

Packaging

1, 5, 25 g in glass bottle

100 mg in glass bottle

Price and Availability


Laboratory Gloves
Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
DV2300000

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles
Peer-Reviewed Papers
15

References

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