39070 Fluka


for the determination of hydroxyproline, ≥99.0% (HPLC)

Synonym: Ehrlich’s reagent


Related Categories Aldehydes, Amines, Amino Acids, Analytical Reagents, Analytical Reagents for General Use,
assay   ≥99.0% (HPLC)
quality   for the determination of hydroxyproline
ign. residue   ≤0.1% (as SO4)
loss   ≤0.5% loss on drying
mp   72-75 °C(lit.)
  73-75 °C
anion traces   chloride (Cl-): ≤50 mg/kg
  sulfate (SO42-): ≤50 mg/kg
cation traces   Ca: ≤10 mg/kg
  Cd: ≤5 mg/kg
  Co: ≤5 mg/kg
  Cr: ≤5 mg/kg
  Cu: ≤5 mg/kg
  Fe: ≤10 mg/kg
  K: ≤50 mg/kg
  Mg: ≤5 mg/kg
  Mn: ≤5 mg/kg
  Na: ≤50 mg/kg
  Ni: ≤5 mg/kg
  Pb: ≤5 mg/kg
  Zn: ≤5 mg/kg



Forms colored condensation products (Schiff bases) with pyrroles1 and primary amines.2

Other Notes

Analytical reagent for the determination of amino acids and peptides, amines, indoles, hydrazines and hydrogen peroxides; spray reagent for tryptophan3,4; For the determination of tryptophan in proteins5; Used in microbiology as test reagent for the identification of indole-producing microorganisms.; For Kovacs reagent dissolve 10 g 4-dimethylamino-benzaldehyde in 150 mL 1-pentanol and add 50 mL hydrochloric acid conc.

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