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49825 Fluka

DMACA Reagent

for microbiology

Synonym: 4-(Dimethylamino)-cinnamaldehyde solution

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Description

Components

Composition per 100 ml:
Dimethylaminocinnamaldehye 1 g
Hydrochloric acid (concentrated) 1.0 ml
distilled water 99.0 ml

Application

Used to determine the ability of an organism to split indole from the tryptophan molecule.

Other Notes

Reagent for the rapid identification of Streptococci.1,2

Price and Availability

Suggested Laboratory Gloves


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Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
UN 1789 8 / PGIII
WGK Germany 
2

Protocols & Articles

Articles

Differentiation of Escherichia coli from coliforms

In August 2008, the discovery of E. coli-contaminated beef in the United States prompted a nationwide recall of beef. The source turned out to be one supplier that had a history of contamination of i...
Jvo Siegrist
AnalytiX Volume 8 Article 5
Keywords: Decarboxylations, Fermentation, Genetic, Indicators, Microbiology, Reductions

Identification of Microorganisms Based on Color

For microbiologists the most fundamental stain was developed in 1884 by the Danish bacteriologist Hans Christian Gram. The Gram Stain allows the determination of morphology, dividing bacteria into tw...
Jvo Siegrist
Microbiology Focus Edition 1.4, Product Manager Microbiology.... ivo.siegrist@sial.com
Keywords: Antibiotics, Enzyme activity, Fermentation, Gram stain, Help, Indicators, Reductions

Pseudomonas Media and Tests

Pseudomonas are motile (one or more polar flagella), rod shaped and aerobe Gram-negative bacteria. They are found almost everywhere, in soil, water, plants and animals. In most cases it is not pathog...
Jvo Siegrist, Product Manager Microbiology, ivo.siegrist@sial.com
Analytix Volume 2007 Article 5
Keywords: Anaerobic, Biochemistry, Cosmetics, Diagnostic, Fermentation, Glycolysis, Metabolism, Microbiology, Pentose phosphate pathway, Pharmaceutical, Respiratory

Peer-Reviewed Papers

References

Set your institution to view full text papers.

1. L.P. Gordon et al. J. Clin. Microbiol. 2, 238, (1987)

Multi-laboratory validation of a standard method for quantifying proanthocyanidins in cranberry powders. Prior, R.L., et al. J. Sci. Food Agric. 90, 1473-8, (2010)

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Inhibition of nicotinoprotein (NAD+-containing) alcohol dehydrogenase by trans-4-(N,N-dimethylamino)-cinnamaldehyde binding to the active site. Piersma SR, Norin A, de Vries S, et al. J. Protein Chem. 22(5), 457-61, (2003)

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Evidence for reactivity of serine-74 with trans-4-(N,N-dimethylamino)cinnamaldehyde during oxidation by the cytoplasmic aldehyde dehydrogenase from sheep liver. Loomes KM, Midwinter GG, Blackwell LF, et al. Biochemistry 29(8), 2070-5, (1990)

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The Arabidopsis TDS4 gene encodes leucoanthocyanidin dioxygenase (LDOX) and is essential for proanthocyanidin synthesis and vacuole development. Abrahams S, Lee E, Walker AR, et al. Plant J. 35(5), 624-36, (2003)

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Comparison of isolated cranberry (Vaccinium macrocarpon Ait.) proanthocyanidins to catechin and procyanidins A2 and B2 for use as standards in the 4-(dimethylamino)cinnamaldehyde assay. Feliciano, R.P., et al. J. Agric. Food Chem. 60, 4578-85, (2012)

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Identification and biochemical characterization of mutants in the proanthocyanidin pathway in Arabidopsis. Abrahams S, Tanner GJ, Larkin PJ, et al. Plant Physiol. 130(2), 561-76, (2002)

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Densitometric determination of (+)-catechin and (-)-epicatechin by 4-dimethylaminocinnamaldehyde reagent. Glavnik V, Simonovska B, and Vovk I J. Chromatogr. A 1216(20), 4485-91, (2009)

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Aldehyde dehydrogenase. Covalent intermediate in aldehyde dehydrogenation and ester hydrolysis. Blatter EE, Abriola DP, and Pietruszko R Biochem. J. 282 ( Pt 2), 353-60, (1992)

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Comparison of adult corn rootworm (Coleoptera: Chrysomelidae) sampling methods. Whitworth RJ, Wilde GE, Shufran RA, et al. J. Econ. Entomol. 95(1), 96-105, (2002)

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Amino acid substitutions at position 47 of human beta 1 beta 1 and beta 2 beta 2 alcohol dehydrogenases affect hydride transfer and coenzyme dissociation rate constants. Stone CL, Bosron WF, and Dunn MF J. Biol. Chem. 268(2), 892-9, (1993)

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Evaluation of parameters that affect the 4-dimethylaminocinnamaldehyde assay for flavanols and proanthocyanidins. Wallace TC and Giusti MM J. Food Sci. 75(7), C619-25, (2010)

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Determination of total procyanidins in selected chocolate and confectionery products using DMAC. Payne MJ, Hurst WJ, Stuart DA, et al. J. AOAC Int. 93(1), 89-96, (2010)

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Use of stains to detect fingermarks. Becue A, Moret S, Champod C, et al. Biotech. Histochem. 86(3), 140-60, (2011)

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Determination of sulfadoxine concentrations in whole blood using C18 solid-phase extraction, sodium dodecyl sulfate and dimethylaminocinnamaldehyde. Green MD, Mount DL, and Todd GD Analyst 120(10), 2623-6, (1995)

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Chemical enhancement of footwear impressions in urine on fabric. Farrugia KJ, Bandey H, Bleay S, et al. Forensic Sci. Int. 214(1-3), 67-81, (2012)

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Novel substrates of yeast alcohol dehydrogenase-3. 4-dimethylamino-cinnamaldehyde and chloroacetaldehyde. Leskovac V, Trivić S, Zeremski J, et al. Biochem. Mol. Biol. Int. 43(2), 365-73, (1997)

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Photophysics of 4-(N,N-dimethylamino)cinnamaldehyde/ alpha-cyclodextrin inclusion complex. Panja S and Chakravorti S Spectrochim. Acta. A. Mol. Biomol. Spectrosc. 58(1), 113-22, (2002)

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Binding and incorporation of 4-trans-(N,N-dimethylamino) cinnamaldehyde by aldehyde dehydrogenase. Dryjanski M, Lehmann T, Abriola D, et al. J. Protein Chem. 18(6), 627-36, (1999)

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A selective and sensitive kinetic method for the determination of procaine and benzocaine in pharmaceuticals. Carmona M, Silva M, and Pérez-Bendito D J. Pharm. Biomed. Anal. 10(2-3), 145-52, (1992)

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Catalysis of dehydrogenation of 4-trans-(N,N-dimethylamino)cinnamaldehyde by aldehyde dehydrogenase. Pietruszko R, Lehmann T, Dryjanski M, et al. Chem. Biol. Interact. 130-132(1-3), 103-14, (2001)

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p-dimethylaminocinnamaldehyde derivatization for colorimetric detection and HPLC-UV/vis-MS/MS identification of indoles. Porubsky PR, Scott EE, and Williams TD Arch. Biochem. Biophys. 475(1), 14-7, (2008)

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Nuclei of plants as a sink for flavanols. Feucht W and Polster J Z. Naturforsch. C 56(5-6), 479-81, (2001)

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Forensic identification of urine using the DMAC test: a method validation study. Ong SY, Wain A, Groombridge L, et al. Sci. Justice 52(2), 90-5, (2012)

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An environmentally friendly reflectometric method for bumetanide determination in pharmaceuticals. Pollo F, Sequinel R, Rufino JL, et al. Anal. Sci. 25(7), 897-901, (2009)

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Aldehyde dehydrogenase: aldehyde dehydrogenation and ester hydrolysis. Pietruszko R, Abriola DP, Blatter EE, et al. Adv. Exp. Med. Biol. 328, 221-31, (1993)

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2. J.F. MacFaddin Biochemical Tests for Identification of Medical Bacteria, (2000)

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