|Related Categories||A - M, All Food Control Media (alphabetical), All Solid Media/Agars, Chromogenic Media, Enterobacteriaceae,|
|shelf life||(limited shelf life, expiry date on the label)|
|composition||agar, 13.5 g/L|
|B.C. indicator, 0.1 g/L|
|bile salts mixture, 1.5 g/L|
|casein enzymic hydrolysate, 17 g/L|
|crystal violet, 0.001 g/L|
|neutral red, 0.03 g/L|
|proteose peptone, 3 g/L|
|sodium chloride, 5 g/L|
|sorbitol, 10 g/L|
|final pH||7.1±0.3 (25 °C)|
|suitability||selective and differential for Enterobacteriaceae|
|selective and differential for Enterovirulent E. coli|
|selective and differential for Escherichia coli and Coliforms|
HiCrome™ MacConkey Sorbitol Agar is recommended for selective isolation of Escherichia coli 0157:H7 from food and animal feeding stuffs. MacConkey Sorbitol Agar is based on the formulation described by Rappaport and Henigh. The medium contains sorbitol instead of lactose and it is recommended for the detection of enteropathogenic strains of E. coli 0157:H7 which ferments lactose but does not ferment sorbitol and hence produce colorless colonies. Sorbitol fermenting strains of E. coli produce pink-red colonies. The red color is due to production of acid from sorbitol, absorption of neutral red and a subsequent color change of the dye when pH of the medium falls below 6.8. E. coli 0157:H7 has been recognised as a cause of haemorrhagic colitis. B.C. indicator is added to detect the presence of an enzyme β-
Suspend 50.1 g in 1 liter of distilled water. Boil gently to dissolve the medium completely. DO NOT AUTOCLAVE. Cool to 50 °C. Mix well and pour into sterile petri plates. If desired add Tellurite-Cefixime Supplement to the molten and cooled medium (50 °C) before pouring into sterile petri plates.
HiCrome is a trademark of HiMedia Laboratories Pvt. Ltd.
Certificate of Analysis
The use of traditional versus improved media formulation containing chromogenic substrate is currently an important topic in the field of microbiology. The focus behind such developments was to produ...
Jvo SiegristProduct Manager
AnalytiX Issue 3
Keywords: Culture media, Diffusion, Electron capture detector, Fermentation, Filtration, Metabolites, Microbiology
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