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Trizma® base

BioUltra, ≥99.8% (T)

DOWNLOAD MSDS (PDF)

Synonym: 2-Amino-2-(hydroxymethyl)-1,3-propanediol THAM Tris base Tris(hydroxymethyl)aminomethane Trometamol

  • CAS Number 77-86-1

  • Linear Formula NH2C(CH2OH)3

  • Molecular Weight 121.14

  •  Beilstein Registry Number 741883

  •  EC Number 201-064-4

  •  MDL number MFCD00004679

  • Popular Documents:  Datasheet (PDF)  

Trizma® base BioUltra, ≥99.8% (T)
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Properties

Related Categories Alphabetical, BioUltra Buffers, Biological Buffers, Zwitterionic
description   aminopeptidase substrate
product line   BioUltra
assay   ≥99.8% (T)
total impurities   insoluble matter, passes filter test
ign. residue   ≤0.01% (as SO4)
loss   ≤0.5% loss on drying, 110 °C
pH   10.5-12.0 (25 °C, 4 M in H2O)
useful pH range   7 - 9
pKa (25 °C)   8.1
bp   219-220 °C/10 mmHg(lit.)
mp   167-172 °C(lit.)
  168-172 °C
solubility   H2O: soluble4 M at 20 °C, clear, colorless
anion traces   chloride (Cl-): ≤5 mg/kg
  sulfate (SO42-): ≤5 mg/kg
cation traces   Al: ≤5 mg/kg
  As: ≤0.1 mg/kg
  Ba: ≤5 mg/kg
  Bi: ≤5 mg/kg
  Ca: ≤10 mg/kg
  Cd: ≤5 mg/kg
  Co: ≤5 mg/kg
  Cr: ≤5 mg/kg
  Cu: ≤5 mg/kg
  Fe: ≤5 mg/kg
  K: ≤50 mg/kg
  Li: ≤5 mg/kg
  Mg: ≤5 mg/kg
  Mn: ≤5 mg/kg
  Mo: ≤5 mg/kg
  Na: ≤50 mg/kg
  Ni: ≤5 mg/kg
  Pb: ≤5 mg/kg
  Sr: ≤5 mg/kg
  Zn: ≤5 mg/kg
λ   4 M in H2O
UV absorption   λ: 260 nm Amax: 0.10
  λ: 280 nm Amax: 0.08
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Description

Other Notes

In a high molar concentration for molecular weight determination of peptides and proteins by gel electrophoresis1; Electrophoresis of proteins on cellulose acetate membranes2; Calibration of polyacrylamide gel columns for the separation of oligonucleotides by capillary electrophoresis3; Determination of trace amounts of fluorine from a single sodium carbonate fusion of small geological sample masses4.

The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Legal Information

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

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