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93349 Fluka

Trizma® base

BioUltra, ≥99.8% (T)

Synonym: 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol

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Properties

Related Categories Alphabetical, BioUltra Buffers, Biochemicals and Reagents, Biological Buffers, Zwitterionic More...
description   aminopeptidase substrate
product line   BioUltra
assay   ≥99.8% (T)
impurities   insoluble matter, passes filter test
ign. residue   ≤0.01% (as SO4)
loss   ≤0.5% loss on drying, 110 °C
pH   10.5-12.0 (25 °C, 4 M in H2O)
useful pH range   7 - 9
pKa (25 °C)   8.1
bp   219-220 °C/10 mmHg(lit.)
mp   167-172 °C(lit.)
  168-172 °C
solubility   H2O: soluble4 M at 20 °C, clear, colorless
anion traces   chloride (Cl-): ≤5 mg/kg
  sulfate (SO42-): ≤5 mg/kg
cation traces   Al: ≤5 mg/kg
  As: ≤0.1 mg/kg
  Ba: ≤5 mg/kg
  Bi: ≤5 mg/kg
  Ca: ≤10 mg/kg
  Cd: ≤5 mg/kg
  Co: ≤5 mg/kg
  Cr: ≤5 mg/kg
  Cu: ≤5 mg/kg
  Fe: ≤5 mg/kg
  K: ≤50 mg/kg
  Li: ≤5 mg/kg
  Mg: ≤5 mg/kg
  Mn: ≤5 mg/kg
  Mo: ≤5 mg/kg
  Na: ≤50 mg/kg
  Ni: ≤5 mg/kg
  Pb: ≤5 mg/kg
  Sr: ≤5 mg/kg
  Zn: ≤5 mg/kg
λ   4 M in H2O
UV absorption   λ: 260 nm Amax: 0.10
  λ: 280 nm Amax: 0.08

Description

Other Notes

In a high molar concentration for molecular weight determination of peptides and proteins by gel electrophoresis1; Electrophoresis of proteins on cellulose acetate membranes2; Calibration of polyacrylamide gel columns for the separation of oligonucleotides by capillary electrophoresis3; Determination of trace amounts of fluorine from a single sodium carbonate fusion of small geological sample masses4.

The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

This product has been replaced by 93362-SIGMA | Trizma base BioUltra, for molecular biology, ≥99.8% (T)

Legal Information

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Price and Availability

Suggested Laboratory Gloves


Laboratory GlovesThis substance has been tested against several types of hand protection for CE compliance. Click below to find the recommended gloves for handling this product.



TruPAGE™ Pre-cast Polyacrylamide Gels


TruPAGE™ Pre-cast Polyacrylamide GelsTruPAGE Gels run true every time!

  • . 2-Year Shelf Life
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  • . Higher Capacity Wells


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Safety & Documentation

Safety Information

Personal Protective Equipment 
WGK Germany 
2
RTECS 
TY2900000

Protocols & Articles

Articles

Determination of Water Content in Tris-Hydroxymethylaminomethane Using Karl Fischer Titration

Nitrogen compounds, acid amides and weakly basic amines (heterocyclenes) give no problems. They can be titrated according to the standard procedures. Strongly basic amines alter the pH value and must...
Keywords: Titrations

Determination of Water Content in Tris-Hydroxymethylaminomethane Using Karl Fischer Titration

Nitrogen compounds, acid amides and weakly basic amines (heterocyclenes) give no problems. They can be titrated according to the standard procedures. Strongly basic amines alter the pH value and must...
Keywords: Titrations

Peer-Reviewed Papers

References

Set your institution to view full text papers.

1. Peptide and protein molecular weight determination by electrophoresis using a high-molarity tris buffer system without urea. Fling, S.P., Gregerson, D.S. Anal. Biochem. 155, 83, (1986)

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2. Electrophoresis of proteins on cellulose acetate membranes. J. Ambler, M. Rodgers Clin. Chem. 26, 1221, (1980)

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3. Calibration of polyacrylamide gel columns for the separation of oligonucleotides by capillary electrophoresis. A. Paulus Electrophoresis 11, 702, (1990)

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4. Kinetic determination of iodide in pharmaceutical and food samples. A. Hofstetter et al. Analyst 116, 65, (1991)

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A rhenium tris-carbonyl derivative as a single core multimodal probe for imaging (SCoMPI) combining infrared and luminescent properties. Clède S, Lambert F, Sandt C, et al. Chem. Commun. (Camb.) 48(62), 7729-31, (2012)

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Zinc binding ligands and cellular zinc trafficking: apo-metallothionein, glutathione, TPEN, proteomic zinc, and Zn-Sp1. Ujala Rana et al J. Inorg. Biochem. 102, 489-99, (2008)

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DCAP: A Broad-Spectrum Antibiotic That Targets the Cytoplasmic Membrane of Bacteria. Eun, Y.J., et al. J. Am. Chem. Soc. 134, 11322-11325, (2012)

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Quantifying the energetic contributions of desolvation and π-electron density during translesion DNA synthesis. Edward A. Motea et al Nucleic Acids Res. 39, 1623-37, (2011)

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A predictive ligand-based Bayesian model for human drug-induced liver injury. Sean Ekins et al Drug Metab. Dispos. 38, 2302-8, (2010)

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The vital role of buffer anions in the antioxidant activity of CeO2 nanoparticles. Xue Y, Zhai Y, Zhou K, et al. Chemistry 18(35), 11115-22, (2012)

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Extraction of membrane proteins by differential solubilization for separation using two-dimensional gel electrophoresis. Molloy, M.P., et al. Electrophoresis 19, 837, (1998)

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Morphine sulfate, transdermal fentanyl citrate and ketorolac tromethamine: effects on postoperative pulmonary function. Mason HH Am. J. Crit. Care 2(1), 61-4, (1993)

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[Ketorolac tromethamine--with special reference to postoperative pain therapy]. Andersen SS and Jensen ME Ugeskr. Laeg. 156(8), 1160-3, (1994)

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Elimination pathways of cyclosarin (GF) mediated by β-cyclodextrin in vitro: pharmacokinetic and toxicokinetic aspects. Kranawetvogl A, Schüler J, Müller S, et al. Toxicol. Lett. 222(2), 164-70, (2013)

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[The use of prostaglandin F2alpha (Prostin 15M) for terminationof second trimester pregnancy]. Bojinova S and Porozhanova K Akush. Ginekol. (Sofiia.) 52(2), 53-5, (2013)

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[Treatment and metaphylaxis of gout complicated by nephropathy and urolithiasis]. Avdoshin VP, Andriukhin MI, Annenkov AV, et al. Urologiia. (5), 18-20, (2012)

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Micellar electrokinetic chromatographic method for mianserin hydrochloride and analysis of degradation products by mass spectrometry. Sfair LL, Gobetti C, Sangoi Mda S, et al. Chem. Pharm. Bull. 60(11), 1387-94, (2012)

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[Treatment and metaphylaxis of urate and mixed urolithiasis]. Avdoshin VP, Andriukhin MI, Israfilov MN, et al. Urologiia. (3), 7-8, 10, (2012)

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Ketorolac tromethamine pharmacokinetics and metabolism after intravenous, intramuscular, and oral administration in humans and animals. Mroszczak EJ, Jung D, Yee J, et al. Pharmacotherapy 10(6 ( Pt 2)), 33S-39S, (1990)

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Dexketoprofen trometamol: clinical evidence supporting its role as a painkiller. Rodríguez MJ, Arbós RM, and Amaro SR Expert Rev. Neurother. 8(11), 1625-40, (2008)

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Ketorolac tromethamine. Jay GT Conn. Med. 55(5), 288-90, (1991)

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Membrane proteins and proteomics: un amour impossible? Santoni, V., et al. Electrophoresis 21, 1054, (2000)

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Fast and scalable purification of a therapeutic full-length antibody based on process crystallization. Smejkal B, Agrawal NJ, Helk B, et al. Biotechnol. Bioeng. 110(9), 2452-61, (2013)

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Novel chemosensor for the visual detection of copper(II) in aqueous solution at the ppm level. Chandrasekhar V, Das S, Yadav R, et al. Inorg. Chem. 51(16), 8664-6, (2012)

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Developing structure-activity relationships for the prediction of hepatotoxicity. Nigel Greene et al Chem. Res. Toxicol. 23, 1215-22, (2010)

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Dissolution patterns of biocompatible glasses in 2-amino-2-hydroxymethyl-propane-1,3-diol (Tris) buffer. Fagerlund S, Hupa L, and Hupa M Acta Biomater. 9(2), 5400-10, (2013)

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Enhanced transfection efficiency of poly(N,N-dimethylaminoethyl methacrylate)-based deposition transfection by combination with tris(hydroxymethyl)aminomethane. Iwai R, Haruki R, Nemoto Y, et al. Bioconjug. Chem. 24(2), 159-66, (2013)

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Electrokinetic supercharging preconcentration prior to CGE analysis of DNA: sensitivity depends on buffer viscosity and electrode configuration. Ye X, Mori S, Yamada M, et al. Electrophoresis 34(4), 583-9, (2013)

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Clinical pharmacokinetics of ketorolac tromethamine. Brocks DR and Jamali F Clin. Pharmacokinet. 23(6), 415-27, (1992)

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Intensive buffering can keep pH above 7.2 for over 4 h during apnea: an experimental porcine study. Höstman S, Engström J, Hedenstierna G, et al. Acta Anaesthesiol. Scand. 57(1), 63-70, (2013)

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Antimicrobial potentiation of irrigation solutions containing tris-[hydroxymethyl] aminomethane-EDTA. Ashworth CD and Nelson DR J. Am. Vet. Med. Assoc. 197(11), 1513-4, (1990)

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Solvent selection and optimization of α-chymotrypsin-catalyzed synthesis of N-Ac-Phe-Tyr-NH2 using mixture design and response surface methodology. Hu SH, Kuo CH, Chang CM, et al. Biotechnol. Prog. 28(6), 1443-9, (2012)

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Tris(hydroxymethyl)aminomethane-functionalized agarose particles: parameters affecting the binding of bovine serum albumin. Zhang B, Wang Y, Gao M, et al. J. Sep. Sci. 35(12), 1406-10, (2012)

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The structural domains of Pseudomonas aeruginosa phosphorylcholine phosphatase cooperate in substrate hydrolysis: 3D structure and enzymatic mechanism. Infantes L, Otero LH, Beassoni PR, et al. J. Mol. Biol. 423(4), 503-14, (2012)

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Fosfomycin tromethamine: single-dose treatment of acute cystitis. Stein GE Int. J. Fertil. Womens. Med. 44(2), 104-9, (1999)

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Fosfomycin tromethamine. A review of its antibacterial activity, pharmacokinetic properties and therapeutic efficacy as a single-dose oral treatment for acute uncomplicated lower urinary tract infections. Patel SS, Balfour JA, and Bryson HM Drugs 53(4), 637-56, (1997)

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Comparative experimental study of myocardial protection with crystalloid solutions for heart transplantation. Lima ML, Fiorelli AI, Vassallo DV, et al. Rev. Bras. Cir. Cardiovasc. 27(1), 110-6, (2012)

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The mechanism of antibacterial activity of tetrandrine against Staphylococcus aureus. Lee YS, Han SH, Lee SH, et al. Foodborne Pathog. Dis. 9(8), 686-91, (2012)

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Prestorage in ovo injection of biological buffers: an approach to improve hatchability in long-term stored eggs. Akhlaghi A, Jafari Ahangari Y, Hashemi SR, et al. Poult. Sci. 92(4), 874-81, (2013)

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Tris(hydroxymethyl) aminomethane salt of ramipril: synthesis, structural characterization from X-ray powder diffraction and stability studies. Bhattacharya A, Chattopadhyay B, Chakraborty S, et al. J. Pharm. Biomed. Anal. 70, 280-7, (2012)

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Acidic infusion in early reperfusion affects the activity of antioxidant enzymes in postischemic isolated rat heart. Penna C, Perrelli MG, Tullio F, et al. J. Surg. Res. 183(1), 111-8, (2013)

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Spectrophotometric determination of drugs with iodine. Karuna T, et al. J. Sci. Ind. Res. 65(10), 808, (2006)

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Cheap, suitable, predictable and manageable nanoparticles for drug delivery: quantum dots. Degim IT and Kadioglu D Curr. Drug Deliv. 10(1), 32-8, (2013)

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Ketorolac tromethamine: an oral/injectable nonsteroidal anti-inflammatory for postoperative pain control. Redden RJ J. Oral Maxillofac. Surg. 50(12), 1310-3, (1992)

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Study of DNA extraction methods for use in loop-mediated isothermal amplification detection of single resting cysts in the toxic dinoflagellates Alexandrium tamarense and A. catenella. Nagai S, Yamamoto K, Hata N, et al. Mar. Genomics 7, 51-6, (2012)

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Deleterious effect of hypothermia in myocardial protection against cold ischemia: a comparative study in isolated rat hearts. Lima ML, Fiorelli AI, Vassallo DV, et al. Transplant. Proc. 44(8), 2326-32, (2012)

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Crystallization and preliminary X-ray diffraction analysis of mevalonate kinase from Methanosarcina mazei. Zhuang N, Seo KH, Chen C, et al. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 68(Pt 12), 1560-3, (2012)

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[Complex therapy of acute and chronic back pain with dexalgin]. Barulin AE and Kurushina OV Zh. Nevrol. Psikhiatr. Im. S. S. Korsakova 112(11), 61-4, (2012)

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THE CLINICAL PHARMACOLOGY OF THAM (TRIS(HYDROXYMETHYL)AMINOMETHANE). NAHAS GG Clin. Pharmacol. Ther. 4, 784-803, (1963)

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Merck 14,9772

Beil. 4,IV,1903

Aldrich MSDS 1, 1815:C / Corp MSDS 1 (2), 3532:A / FT-IR 1 (1), 347:C / FT-IR 2 (1), 542:D / IR-Spectra (3), 203:G / IR-Spectra (2), 183:E / IR-Spectra (2), 183:D / IR-Spectra (3), 203:H / NMR-Reference 2 (1), 304:A / RegBook 1 (1), 371:C / Sax 6, 2558 / Sigma FT-IR 1 (2), 60:D / Sigma FT-IR 1 (2), 61:C / Structure Index 1, 52:C:5 / Vapor Phase 3, 437:C

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