8.51006 EMD Millipore


HBTU 2-(1H-Benzotriazole-1-yl)-1,1,3,3-tetramethyluronium hexafluorophosphate Novabiochem®. CAS 94790-37-1, chemical formula C₁₁H₁₆N₅O*F₆P., 2-(1H-Benzotriazole-1-yl)-1,1,3,3-tetramethyluronium hexafluorophosphate Novabiochem®

Synonym: 2-(1H-Benzotriazole-1-yl)-1,1,3,3-tetramethylaminium hexafluorophosphate

  • CAS Number 94790-37-1

  • Linear Formula C₁₁H₁₆F₆N₅OP

  • Molecular Weight 379.25 g/mol

  •  EC Number 423-020-5



brand family   Novabiochem
packaging   100 g in Plastic bottle (8510060100)
  25 g in Plastic bottle (8510060025)
solubility   1.6 g/l
mp   250 °C
pH   4.1 (1.6 g/l, H₂O)
LD 50 oral   LD50 Rat > 2000 mg/kg
shipped in   ambient
storage temp.   Store at +2°C to +8°C.
specifications   Colour (visual): white to slight yellow to beige
  Appearance of substance (visual): powder
  Identity (IR): passes test
  Assay (HPLC, area%): ≥ 99.0 % (a/a)
  Solubility (1 mmole in 2 ml DMF): clearly soluble


Background information

TBTU and HBTU are two of the most popular in situ activation reagents used in solid phase and solution phase peptide synthesis [1-9]. These reagents offer reactivity similar to symmetrical anhydrides and BOP [1]. Couplings proceed smoothly and rates can even be enhanced by the addition of HOBt [2, 4, 5, 6]. In addition to having high reactivity, TBTU and HBTU have also been shown to limit enantiomerization during fragment condensation and during DMAP catalyzed esterification of arginine derivatives [3]. Comparative experiments between HBTU and TBTU have shown that the counter ion has no influence on coupling rates or levels of enantiomerization [1].

The product number for this product was previously 01-62-0010.

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Safety Information

HS Code 
2933 99 80
Hazard Statement(s) 
H228: Flammable solid.
H315: Causes skin irritation.
H319: Causes serious eye irritation.
Precautionary Statement(s) 
P210: Keep away from heat/sparks/open flames/hot surfaces. No smoking.
P302 + P352: IF ON SKIN: Wash with plenty of soap and water.
P305 + P351 + P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
Storage class 
4.1B Flammable solid hazardous materials
WGK 2 water endangering
Hazard Pictogram(s) 
GHS02  GHS02
Hazard Pictogram(s) 
GHS07  GHS07
Hazard Symbols 
Irritant  Irritant
Declaration (railroad and road) ADR, RID 
UN 1325 , 4.1, II
Declaration (transport by air) IATA-DGR 
UN 1325 , 4.1, II
Declaration (transport by sea) IMDG-Code 
UN 1325 , 4.1, II
Categories of danger 
R Phrase 
R 36/38
Irritating to eyes and skin.
S Phrase 
S 15
Keep away from heat.
Protocols & Articles


Peptide Coupling Reagents Selection Guide

The Novabiochem® brand offers one of the most extensive ranges of high-quality coupling reagents for in situ activation on the market. However, with such a plethora of reagents available, the choice ...
Keywords: Condensations, Coupling reactions, Cyclizations, Racemizations, Solid phase peptide synthesis

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