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11096176001 Roche

Blocking Reagent

For nucleic acid hybridization and detection

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Properties

Related Categories Blocking Reagents for Hybridization, Molecular Biology, Nucleic Acid Detection and Hybridization, Nucleic Acid Hybridization Reagents
form   powder
packaging   pkg of 50 g
mfr. no.   Roche
shipped in   ambient
storage temp.   20-25°C

Description

General description

Download the CofA, SDS and IFU specific to your product lot and format

Other Notes

For life science research only. Not for use in diagnostic procedures.

Preparation Note

Working concentration: Dilution of Antibody 1:5,000
Working solution: To prepare a 10x blocking stock solution Blocking Reagent is dissolved in maleic acid buffer to a final concentration of 10% (w/v) with shaking and heating either on a heating block or in a microwave oven.
Storage conditions (working solution): 10x stock solution of Blocking Reagent is autoclaved and stored at 2 to 8 °C. Alternativly, aliquots can be stored at -15 to -25 °C.

Application

The Blocking Reagent is used to decrease the background in nonradioactive filter hybridization and the detection of nucleic acid hybrids. It has also been used as a blocking agent in immunohistochemistry.

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

Protocols & Articles

Protocols

Blocking Reagent Protocol & Troubleshooting

Preparation of stock solution (10x): Blocking Reagent is dissolved in Maleic acid buffer [100 mM Maleic acid, 250 mM NaCl, pH 7.5 (20 °C) adjust with conc. or solid NaOH], to a final concentration of...
Keywords: Evaporation, In Situ hybridization, Microarray Analysis, Nucleic acid hybridization

Peer-Reviewed Papers
15

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