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11363514910 Roche

DIG Luminescent Detection Kit Green Alternative

Synonym: luminescent detection kit



Related Categories 12 Principles Aligned Products, Chemical Synthesis, Greener Alternative Products, Life Sciences
assay   >98% (NMR)
usage   sufficient for 50 blots (10 cm x 10 cm each)
mfr. no.   Roche
greener alternative product characteristics   Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
shipped in   dry ice
storage temp.   −20°C


General description

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DIG Luminescent Detection Kit is used for the detection of digoxigenin-labeled nucleic acids by enzyme immunoassay with luminescence on nylon membranes.

Sigma Life Science is committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical.  The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.

Other Notes

For life science research only. Not for use in diagnostic procedures.


1 kit containing 5 components.

Preparation Note

Working concentration: 0.5mg/ml for flow cytometry
• Storage conditions (working solution): Antibody conjugate (vial 3): once opened, should be stored at 2 to 8°C
• Blocking Reagent (bottle 4): in solution at 2 to 8°C
• CSPD (vial 5): at 2 to 8°C when frequently used. Repeated freeze/thaw cycles should be avoided.

Enzymatic dephosphorylation of the dioxetane CSPD by alkaline phosphatase leads to the metastable phenolate anion, which decomposes and emits light at 477nm. The light emission increases with time until a constant intensity is attained.
Assay Time
• Immunological detection 1.5 hours
• Signal detection 5 to 30 minute

Note: Store protected from light.


DIG Luminescent Detection Kit is used for highly sensitive and specific detection of DIG-labeled nucleic acids in Southern and Northern blots and plaque or colony lifts with anti-DIG-AP conjugate and the chemiluminescent substrate CSPD. Chemiluminescent detection with CSPD (following the instructions of the kit) is as sensitive as radioactive methods, but requires much shorter exposure times.


Sensitivity: 0.03 pg of homologous DNA or 0.1 pg of homologous RNA is detected in a Southern or dot blot after <30-min exposure time. Single-copy genes are detected in 0.3 μg mammalian genomic DNA.

Price and Availability

Kit component only


Product #

Add to Cart

DIG-labeled Control DNA 5 µg/ml SDS    
DNA Dilution Buffer SDS    
Anti-digoxigenin-AP antibody, Fab fragments 750 U/ml SDS    
Blocking Reagent SDS    
CSPD 11.6 mg/ml SDS    
Safety & Documentation

Safety Information

NONH for all modes of transport


Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers


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