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11370529001 Roche

Endoproteinase Arg-C Sequencing Grade

from Clostridium histolyticum

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Properties

Related Categories Biochemicals and Reagents, Enzymes, Inhibitors, and Substrates, Proteolytic Enzymes, Proteolytic Enzymes and Substrates
form   lyophilized
mol wt   Mr 59 kDa
packaging   pkg of 3 × 5 μg
mfr. no.   Roche
parameter   37 °C optimum reaction temp.
optimum pH   7.2 - 8.0
shipped in   wet ice
storage temp.   2-8°C

Description

Application

Use Endoproteinase Arg-C Sequencing Grade for the specific cleavage of proteins and peptides for peptide mapping, fingerprinting, and sequence analysis. The protease is suitable to digest proteins in solution, in gels, or on blotting membranes.

General description

Download the CofA, SDS and IFU specific to your product lot and format

Endoproteinase Arg-C Sequencing Grade is isolated from Clostridium histolyticum as a highly purified and specific cysteine protease.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Preparation Note

Working concentration: Nutridoma-CS (50x) is diluted 1:50 (v/v) in basal medium. It is strongly suggested to use RPMI 1640. The final protein concentration is less than 1 mg/ml. The final medium should also contain L-glutamine and β-mercaptoethanol.
Inhibitors: Oxidizing agents, sulfhydryl reactants (e.g., TLCK), EDTA, Co2+, Cu2+, Cd2+. Citrate, borate, and Tris anions partially inhibit.
Optimum pH: 7.2 - 8.0
Storage conditions (working solution): -15 to -25°C
For long-term use, the solution should be gently flushed with a stream of nitrogen and stored at -15 to -25°C. The reconstituted incubation buffer is stable for several months at -15 to -25°C.

Quality

Purity: Free of impurities that may interfere with the specific cleavage or separation of peptides in reversed-phase HPLC.

Reconstitution

The lyophilized endoproteinase Arg-C sequencing grade is reconstituted in 50 μl double-dist. water to give a final concentration of 50 mM Tris-HCl buffer, 10 mM CaCl2, 5 mM EDTA, pH 8.0. The lyophilized activation solution is dissolved in 100 μl double-dist. water resulting in a final 50 mM dithiothreitol-(DTT-)concentration and 5 mM EDTA-concentration.

Specificity

Endoproteinase Arg-C Sequencing Grade is a cysteine protease that specifically cleaves peptide, ester, and amide bonds at the C-terminal side of arginine residues. The specificity is primarily to arginine residues, although hydrolysis proceeds to a minor degree in most lysine-containing substrates. Reducing agents (such as DTT) and Ca2+ are required for full activity.
The specificity of Endoproteinase Arg-C Sequencing Grade is tested with the oxidized B-chain of insulin (insulin Box) as a substrate. High concentrations of Endoproteinase Arg-C Sequencing Grade are incubated for prolonged incubation times to detect traces of impurities such as contaminating proteases.
Recognition sites: C-terminal side of arginine residues, other recognition sites with lower efficiency.

Price and Availability


Biomedical Applications
Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
Protocols & Articles
Peer-Reviewed Papers
15

References

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