11383213001 Roche


4-Nitro blue tetrazolium chloride, solution



Related Categories Colorimetric, Molecular Biology, Nucleic Acid Detection Substrates and Reagents, Nucleic Acid Detection and Hybridization
assay   >95%
form   solution
mol wt   Mr 817.7
packaging   pkg of 3 mL (300 mg)
mfr. no.   Roche
shipped in   dry ice
storage temp.   −20°C


Other Notes

For life science research only. Not for use in diagnostic procedures.

Preparation Note

Working solution: Preparation of staining solution
• Always prepare this solution fresh shortly before use!For all applications except DIG System: Add 50 μl NBT solution and 37,5 μl BCIP to 10 ml 0.1 M Tris-HCl, pH 9.5 (20 °C), 0.1 M NaCl, 0.05 M MgCl2.
• For DIG System applications: Add 50 μl NBT solution and 37,5 μl BCIP to 10 ml 0.1 M Tris-HCl, pH 9.5 (20 °C), 0.1 M NaCl
• Note: Do not include MgCl2 in the DIG detection buffer as this might lead to spotty background on the membrane after the detection procedure. Alkaline Phosphatase does not require Mg2+.

Physical form

Solution (100 mg/ml) in 70% dimethylformamide (DMF) (v/v). The color of the NBT solution can vary between yellow and brown depending on the lot used.

Note: The color does not impair the quality or the function of the reagent. In some cases a precipitate may occur which is easily brought into solution by briefly warming the substrate at 37 °C.


• Immunohistocytochemistry
• Western blot
• Southern blot
• Northern blot
• Colony and plaque hybridization
• In situ hybridization

General description

NBT is a chromogenic and soluble substance. It is a substrate of alkaline phosphatase (AP) and is converted to an indigo/purple dye on its action.

Price and Availability

Safety & Documentation

Safety Information

Signal word 
Hazard statements 
NONH for all modes of transport


Certificate of Analysis

Protocols & Articles


NBT Protocol & Troubleshooting

Possible counterstains in combination with BM Purple (or NBT/BCIP in general): There are several counterstains possible in combination with BM Purple (or NBT/BCIP in general), including FastGreen FCF...
Keywords: In Situ hybridization, Nucleic acid hybridization, Reductions

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