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11383213001 Roche

NBT

4-Nitro blue tetrazolium chloride, solution

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Properties

Related Categories Colorimetric, Molecular Biology, Nucleic Acid Detection Substrates and Reagents, Nucleic Acid Detection and Hybridization
assay   >95%
form   solution
mol wt   Mr 817.7
packaging   pkg of 3 mL (300 mg)
mfr. no.   Roche
shipped in   dry ice
storage temp.   −20°C

Description

Application

• Immunohistocytochemistry
• Western blot
• Southern blot
• Northern blot
• Colony and plaque hybridization
• In situ hybridization

General description

Download the CofA, SDS and IFU specific to your product lot and format

Other Notes

For life science research only. Not for use in diagnostic procedures.

Preparation Note

Working solution: Preparation of staining solution
• Always prepare this solution fresh shortly before use!For all applications except DIG System: Add 50 μl NBT solution and 37,5 μl BCIP to 10 ml 0.1 M Tris-HCl, pH 9.5 (20 °C), 0.1 M NaCl, 0.05 M MgCl2.
• For DIG System applications: Add 50 μl NBT solution and 37,5 μl BCIP to 10 ml 0.1 M Tris-HCl, pH 9.5 (20 °C), 0.1 M NaCl
• Note: Do not include MgCl2 in the DIG detection buffer as this might lead to spotty background on the membrane after the detection procedure. Alkaline Phosphatase does not require Mg2+.

Physical form

Solution (100 mg/ml) in 70% dimethylformamide (DMF) (v/v). The color of the NBT solution can vary between yellow and brown depending on the lot used.

Note: The color does not impair the quality or the function of the reagent. In some cases a precipitate may occur which is easily brought into solution by briefly warming the substrate at 37 °C.

Price and Availability


Hybridization

Roche Biochemical Reagents
Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

Protocols & Articles

Protocols

NBT Protocol & Troubleshooting

Possible counterstains in combination with BM Purple (or NBT/BCIP in general): There are several counterstains possible in combination with BM Purple (or NBT/BCIP in general), including FastGreen FCF...
Keywords: In Situ hybridization, Nucleic acid hybridization, Reductions

Peer-Reviewed Papers
15

References

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