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11582950001 Roche

BM Chemiluminescence ELISA Substrate (POD)

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Properties

Related Categories Chemiluminescent, Molecular Biology, Nucleic Acid Detection Substrates and Reagents, Nucleic Acid Detection and Hybridization
form   solution
mfr. no.   Roche
packaging   pkg of 250 mL (for 2500 wells or 1000 tubes)
shipped in   wet ice
storage temp.   2-8°C

Description

Application

The BM Chemiluminescence ELISA Substrate (POD) provides a substrate solution for peroxidase-based (POD, HRP) secondary detection systems with highly sensitive, enhanced chemiluminescence. This reagent has been optimized for ELISA applications to be run on a microplate chemiluminescence reader (96-well format) or tube-format luminometers.

Features and Benefits

• Improved sensitivity
• Large dynamic range
• Rapid and constant signal

Contents
Substrate Reagent A (buffered solution that contains luminol/4-iodophenol)
Starting Reagent B (buffered solution that contains a stabilized form of H2O2)

General description

Download the CofA, SDS and IFU specific to your product lot and format

The sensitivity of enzyme-linked immunosorbent assays often depends on the detection limit of the colorimetric substrates used. In addition, the dynamic range of a colorimetric assay is restricted to a maximum of two orders of magnitude due to the physical/chemical limitations of absorbance measurement (Law of Lambert & Beer). With chemiluminescence technology, a nonradioactive method, which combines the convenience of tube or microplate-based immunoassays with the advantages of isotopic assays, is now available. The BM Chemiluminescence ELISA Substrate (POD) has been evaluated with different immunoassays on the LB 96 P microplate reader and the LB 953 tube chemiluminescence analyzer from EG&G;Berthold.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Principle

Horseradish peroxidase (POD, HRP), in the presence of hydrogen peroxide (H2O2), catalyzes the oxidation of diacylhydrazides such as luminol. A reaction product in an excited state is thus formed, then decays to the ground state by emitting light. Strong enhancement of the light emission is achieved by the agent 4-iodophenol (contained), which acts as a radical transmitter between the formed oxygen radical and luminol.

Preparation Note

Working solution: Preparation of working solution

The BM Chemiluminescence ELISA Substrate is supplied as a set of two stable solutions. Depending on the scale of the assay, the appropriate amount of substrate solution has to be prepared 15 minutes before use: • Add to 100 parts of solution A one part of solution B.
• Stir the mixture for at least 15 minutes at 15 to 25 °C to equilibrate the components.
Premixed working solution stored at 2 to 8 °C should be warmed up to 15 to 25 °C to avoid temperature effects during measurement.
Storage conditions (working solution): The premixed solution is stable for 1 week, when stored at 2 to 8 °C.

Price and Availability


Hybridization

Roche Biochemical Reagents
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

Protocols & Articles

Articles

BM Chemiluminescence ELISA Substrate (POD) Troubleshooting

Optimizing ELISA protocols for chemiluminescence detection POD-conjugate concentration First use the conjugate concentration that is recommended or has been optimized for the individual assay. When t...
Keywords: Buffers, Detergents, Enzyme-linked immunosorbent assay

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