• USA Home
  • 2GFHSRMKB - KAPA2G Fast HotStart ReadyMix

EMAIL THIS PAGE TO A FRIEND
2GFHSRMKB Roche

KAPA2G Fast HotStart ReadyMix

Purchase

Properties

Related Categories Hot Start PCR, Molecular Biology, PCR/Amplification More...
shelf life   ≤18 mo.
packaging   kit of 1.25 mL (100 x 25 μL rxn; KK5603)
  kit of 6.25 mL (500 x 25 μL rxn; KK5601)
shipped in   dry ice
storage temp.   −20°C

Description

Packaging

• KAPA2G Fast Standard or HotStart DNA Polymerase (5 U/μL)
• 5X KAPA2G Buffer A with MgCl2
• 5X KAPA2G Buffer M (Mg-free; available with select HotStart kits)
• MgCl2 (25 mM)
• dNTP Mix (10 mM each dNTP; optional)

Preparation Note

Always ensure that the product has been fully thawed and mixed before use. Reagents may be stored at 4°C for short-term use (up to 1 month). Return to -20°C for long-term storage. Provided that all components have been handled carefully and not contaminated, the kit is not expected to be compromised if left (unintentionally) at room temperature for a short period of time (up to 3 days). Long-term storage at room temperature and 4°C is not recommended. Please note that reagents stored at temperatures above -20°C are more prone to degradation when contaminated during use, and therefore storage at such temperatures is at the user’s own risk.

Quality

Each batch of KAPA2G Fast DNA Polymerase is confirmed to contain <2% contaminating protein (Agilent Protein 230 Assay). KAPA2G Fast ReadyMix is subjected to stringent quality control tests, is free of contaminating exo- and endonuclease activity, and meets strict requirements with respect to DNA contamination levels.

Application

• Routine PCR
• Fast PCR
• Genotyping

Features and Benefits

Reduce cycling times up to 75% :
• Extension times as low as 1 sec per kilobase
• Broad coverage of both AT- and GC-rich targets

High speed, higher performance :

• Single copy sensitivity from complex targets
• Increased yields

Quick Notes :
• KAPA2G Fast HotStart ReadyMix PCR Kits contain the engineered KAPA2G Fast HotStart DNA Polymerase, developed for fast PCR.
• Use 1 sec extension time for amplicons <1 kb and 15 sec/kb for longer amplicons, and save 20–70% of total reaction time.
• No need for specialized instrumentation or PCR consumables.
• Optimized buffer system offers improved yields, specificity and sensitivity, facilitating efficient primer annealing across a wide range of primer lengths, GC contents, and melting temperatures.
• For high reaction efficiency, do not exceed 25 μL reaction volumes.
• KAPA2G Fast HotStart ReadyMix contains 1.5 mM MgCl2 at 1X.
• KAPA2G Fast HotStart ReadyMix with dye includes two inert tracking dyes to allow direct loading onto agarose gels.Reaction products are 3’-dA-tailed and may be cloned into TA cloning vectors.

General description

KAPA2G Fast DNA Polymerase is a second-generation (2G) enzyme engineered for higher processivity and speed, offering significantly faster extension rates than wild-type Taq polymerase. In addition to speed, KAPA2G Fast provides higher yields and sensitivity than competitor enzymes across a broad range of targets.
KAPA2G Fast DNA Polymerase Kits are designed for fast PCR, in which total reaction times are 20–70% shorter than those of conventional PCR assays performed with wild-type Taq DNA polymerase. This can be achieved without sacrificing reaction performance, and does not require specialized PCR consumables or thermocyclers.

Other Notes

For Research Use Only. Not for use in diagnostic procedures.

Price and Availability

Safety & Documentation

Safety Information

Symbol 
GHS08  GHS08
Signal word 
Warning
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

Protocols & Articles

Articles

KAPA2G Fast PCR Kits FAQ

What is Fast PCR? Fast PCR is PCR in which total reaction time is decreased by reducing the duration of one or more of the steps during each cycle of a PCR. Using the KAPA2G Fast PCR Kit, PCR time ca...
Keywords: AGE, Amplification, Buffers, Cloning, Detergents, Electrophoresis, Gel electrophoresis, Genetic, Nucleic acid annealing, Nucleic acid denaturation, Polymerase chain reaction, Purification, Reductions, Sequencing, Size-exclusion chromatography

Related Content

PCR Selection Guide

Sigma-Aldrich offers a wide variety of PCR reagents to meet any experimental needs. Our range of polymerases is customized to meet your End-Point PCR, qPCR, or RT-PCR needs. Our products vary from ro...
Keywords: Polymerase chain reaction, Polymerase chain reaction - quantitative

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?