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Liberase TM Research Grade

medium Thermolysin concentration



form   lyophilized
packaging   pkg of 10 mg (05401119001 [2 x 5 mg])
  pkg of 100 mg (05401127001 [2 x 50 mg])
mfr. no.   Roche
limit   35-37 °C optimum reaction temp.
optimum pH   7.4
shipped in   dry ice
storage temp.   −20°C


Features and Benefits

• Maximize viability and yield of isolated cells: with an enzyme blend that has less clostripain and trypsin activity, as well as reduced endotoxin content.
• Count on higher specific activity of the enzyme blend: as a result of higher Collagenase I + II purity (determined by HPLC analysis).
• Obtain higher experimental reproducibility: due to higher lot-to-lot consistency.
• Increase safety: with an enzyme that is free of any mammalian or avian tissue-derived raw materials.

General description

Download the CofA, SDS and IFU specific to your product lot and format

Liberase TM Research Grade contains highly purified Collagenase I and Collagenase II. These two collagenase isoforms are blended in a precise ratio to each other, and with a medium concentration of Thermolysin, a non-clostridial neutral protease.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Preparation Note

Stabilizers: Calcium
Working concentration: Liberase Research Grade Enzyme Working Concentration

Liberase enzymes have significantly higher specific activities than traditional collagenases. This means that the working concentration of Liberase Research Grade Purified Enzymes, expressed in mg/ml, will be lower than that of traditional collagenase.When the application is on the Roche list of applications at www.collagenase.com, use the Liberase Research Grade concentration recommended for that application.
When the application is not included on this list, first use Liberase TM Research Grade at a concentration of 0.08–0.28 Wünsch units/ml.The goal is to determine the best starting concentration of Liberase Research Grade Enzyme Blends. This is a starting point, and the final concentration may vary due to differences in procedure and lot-to-lot differences in traditional collagenase.

Collagenase Working Concentration

Multiply your previous collagenase working concentration (mg/ml) by its specific activity (Wünsch units/mg, [as determined above]), to obtain Wünsch units/ml. To determine how much Liberase Research Grade Enzyme Blend to use, first multiply your collagenase working concentration (in Wünsch units/ml) times the total volume of your working enzyme solution to obtain the total collagenase activity needed (Wünsch units). Divide the total collagenase activity required by the Liberase Research Grade stock concentration ( “Reconstitution and Storage”). This indicates how many milliliters of Liberase Research Grade Enzyme Blend stock solution to use in your working enzyme solution.
Storage conditions (working solution): Store unused stock solution in single-use aliquots at -15 to -25 °C. For further information on product stability, please visit the Roche Liberase Enzyme website at www.collagenase.com.

Note: Avoid repeated freezing and thawing!


Reconstitute the lyophilized enzyme with injection-quality sterile water or tissue-dissociation buffer. Do not add serum or other components, such as albumin or protease inhibitors, to the dissociation buffer. Enzyme stability is reduced at higher concentrations and warmer temperatures (4 °C). Avoid both the above conditions.

Reconstitute the entire vial. Do not weigh individual aliquots of the lyophilizate. The introduction of moisture into the vial results in a decline in enzymatic activity.

Place vial on ice to rehydrate the lyophilized enzyme.

Gently agitate the vial at 2 to 8 °C until enzyme is completely dissolved (max. 30 min).

Depending on the type of tissue-dissociation buffer used to dissolve Liberase Research Grade Purified Enzyme Blends, slight precipitations may be observed which readily dissolve in the dilutedworking solution, and have no influence on enzyme activity.

Remove an aliquot of the stock solution to prepare the working solution.

Reconstitution volume

2 ml (1 vial with 5 mg–10 mg pack size), 10 ml (1 vial with 50 mg–100 mg pack size)

Collagenase Wünsch (units/ml)

13 (1 vial with 5 mg–10 mg pack size), 26 (1 vial with 50 mg–100 mg pack size)

Total Collagenase concentration [mg/ml]

2.5 (1 vial with 5 mg–10 mg pack size), 5.0 (1 vial with 50 mg–100 mg pack size)


Liberase(Thermolysin Medium) Research Grade is used for the dissociation of a broad range of tissue types, where high purity of the enzyme blend is necessary for high cell yield and viability. Bacterial by-products, such as endotoxins, are reduced up to several thousand-fold.

Legal Information

Liberase is a trademark of Roche

Price and Availability

Refer a colleague - New Lab Start-Up program
Safety & Documentation

Safety Information

Signal word 
Hazard statements 
NONH for all modes of transport


Certificate of Analysis

Protocols & Articles


Liberase™ TM Research Grade Protocol & Troubleshooting

Selection of the appropriate Liberase Research Grade Enzyme Blend Your current tissue dissociation protocol, and the extent to which you have been successful with traditional collagenase are good gui...
Keywords: Cell culture, Cell disruption, Cell dissociation, Coagulation, Enzyme activity, High performance liquid chromatography, Vitamins

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