• USA Home
  • ENDOGLUS-RO - Endoproteinase Glu-C Sequencing Grade

EMAIL THIS PAGE TO A FRIEND
ENDOGLUS-RO Roche

Endoproteinase Glu-C Sequencing Grade

from Staphylococcus aureus V8

Purchase

Properties

Related Categories Biochemicals and Reagents, Enzymes, Inhibitors, and Substrates, Proteolytic Enzymes, Proteolytic Enzymes and Substrates
form   lyophilized (salt-free)
  lyophilized
specific activity activity   ≥15 units/mg protein
mol wt   Mr 27 kDa
packaging   pkg of 3 × 50 μg (11047817001)
  pkg of 50 μg (11420399001)
mfr. no.   Roche
optimum pH   4.0 - 7.8
shipped in   wet ice
storage temp.   2-8°C

Description

Features and Benefits

Free of impurities that may interfere with separation of peptides in RP HPLC.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Preparation Note

Working concentration: 1/100 to 1/20 (w/w)
With a minimum amount of 1 to 5 μg insulin Box, sufficient levels can be detected using the Column Aquapore RUP 300, 4.6 x 100 nm, 7 μm.
Working solution: Solvent is recommended in double-distilled water.
Solvents other than water have not been tested; Roche recommends using 50 mM Hepes, pH 7.8.
Storage conditions (working solution): -15 to -25 °C
At least 2 months if stored frozen in portions.
Stability: At +2 to +8°C within specification range for 12 months; stored dry.

Reconstitution

Lyophilized Endoproteinase Glu-C sequencing grade is reconstituted in double-dist. water. To avoid autolysis, the incubation temperature should not exceed 25 °C.

Specificity

Serine protease that very specifically hydrolyzes peptide bonds at the carboxylic side of Glu (in ammonium bicarbonate, pH 7.8, or ammonium acetate buffer, pH 4.0), or Glu and Asp (in phosphate buffer, pH 7.8). The specificity of Endoproteinase Glu-C Sequencing Grade is verified with the oxidized B-chain of insulin (insulin Box) as substrate. High concentrations of Endoproteinase Glu-C Sequencing Grade (1 part by weight enzyme with 10 parts by weight insulin Box) are incubated to detect traces of impurities, for example, contaminating proteases.
Heat inactivation: Most proteases sequencing grade are stopped by boiling for 5 minutes.

Application

Use Endoproteinase Glu-C Sequencing Grade for protein structure analysis and for sequence analysis.

General description

Endoproteinase Glu-C Sequencing Grade is a serine protease isolated from Staphylococcus aureus V8 as a highly purified and specific protease. Inhibitors of Endoproteinase Glu-C are DFP, α2-macroglobulin and TLCK.

Price and Availability


Biomedical Applications
Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
RIDADR 
NONH for all modes of transport
Protocols & Articles
Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?