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HSMGTKB Roche

KAPA HotStart Mouse Genotyping Kit

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Properties

Related Categories Genomic DNA Amplification, Molecular Biology, PCR/Amplification, from Mammalian Tissue
usage   sufficient for 100 reactions (KK7351)
  sufficient for 500 reactions (KK7352)
shelf life   ≤18 mo.
shipped in   dry ice
storage temp.   −20°C

Description

Packaging

• 2X KAPA2G Fast Standard or HotStart Genotyping Mix with dye
• KAPA Express Extract (1 U/μL)
• 10X KAPA Express Extract Buffer

Quality

All components contained with KAPA Mouse Genotyping Kits are subjected to stringent quality control tests, are free of contaminating exo and endonuclease activities and meet strict requirements with respect to DNA contamination.

Application

• Extraction and amplification from mouse tail, ear, and toe tissue
• Extraction and amplification of DNA from other animal tissue

Always ensure that the product has been fully thawed and mixed before use. Reagents may be stored at 4°C for short-term use (up to 1 month). Return to -20°C for long-term storage. Provided that all components have been handled carefully and not contaminated, the kit is not expected to be compromised if left (unintentionally) at room temperature for a short period of time (up to 3 days).

Features and Benefits

Increased throughput, turnaround time and reliability
• PCR-ready DNA generated in as little as 15 minutes.
• Minimal handling, thereby reducing the risk of sample loss or contamination.
• Sufficient template for multiple assays; easily scaled to handle samples in a 96-well format.
• Fast and reliable amplification of DNA fragments across a wide range of amplicon lengths and GC contents.

Outperforms crude extraction methods and commercial kits
• Increased turnaround times
• Increased specificity
• Decreased cost

Quick Notes:
• Extract PCR-ready DNA from mouse tail, ear, or toe tissue in 15 minutes.
• Dilute DNA extract 10-fold prior to PCR in 10 mM Tris-HCl (pH 8.0–8.5), and use 1 μL of DNA per 25 μL reaction.
• Extracts are stable at -20°C for >6 months.
• KAPA2G Fast (HotStart) Genotyping Mix contains the engineered KAPA2G Fast DNA Polymerase for fast PCR, along with buffer, MgCl2 (1.5 mM at 1X), and dNTPs (0.2 mM each at 1X).
• Loading dye for electrophoresis is included in the KAPA2G Fast Genotyping Mix, which allows direct loading of PCR products onto agarose gels for analysis.
• Use 15 sec annealing time, with 15 sec extension time for amplicons <1 kb, or 15–30 sec/kb extension for longer amplicons.

General description

KAPA Mouse Genotyping Kits include KAPA Express Extract, a novel thermostable protease and buffer system that allows for the extraction of PCR-ready DNA from mouse tissue in as little as 15 minutes, and KAPA2G Fast Genotyping Mix with dye, containing a DNA polymerase engineered via directed evolution for high processivity and extreme speed. The combination of KAPA Express Extract and KAPA2G Fast Genotyping Mix allows for the reliable extraction and amplification of DNA fragments from mouse tissue in as little as 1 hour, as compared to ≥1 day with conventional protocols.

Other Notes

For Research Use Only. Not for use in diagnostic procedures.

Price and Availability

Safety & Documentation

Safety Information

Symbol 
GHS08  GHS08
Signal word 
Warning
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

Protocols & Articles

Articles

KAPA Mouse Genotyping Kit FAQs

What are the recommended applications for the KAPA Mouse Genotyping Kit? KAPA Mouse Genotyping Kits are ideally suited for the rapid extraction and amplification of DNA from mouse tail, ear and toe t...
Keywords: Amplification, Cell disruption, Centrifugation, Cloning, Digestions, Electrophoresis, Nucleic acid annealing, Nucleic acid denaturation, Polymerase chain reaction, Polymerase chain reaction - quantitative, Purification, Size-exclusion chromatography, Solvents

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PCR Selection Guide

Sigma-Aldrich offers a wide variety of PCR reagents to meet any experimental needs. Our range of polymerases is customized to meet your End-Point PCR, qPCR, or RT-PCR needs. Our products vary from ro...
Keywords: Polymerase chain reaction, Polymerase chain reaction - quantitative

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