• USA Home
  • SF1UKB - KAPA SYBR® FAST One-Step

EMAIL THIS PAGE TO A FRIEND
SF1UKB Roche

KAPA SYBR® FAST One-Step

Universal

Purchase

Properties

Related Categories Molecular Biology, PCR/Amplification, RT-PCR and RT-qPCR More...
shelf life   ≤6 mo.
packaging   kit of 1 mL (100 x 20 μL rxn; KK4650)
  kit of 10 mL (1000 x 20 μL rxn; KK4652)
  kit of 5 mL (500 x 20 μL rxn; KK4651)
shipped in   dry ice
storage temp.   −20°C

Description

Packaging

Kits include KAPA RT Mix, dUTP, and KAPA SYBR FAST qPCR Master Mix, which contains KAPA SYBR FAST DNA Polymerase, reaction buffer, dNTPs, SYBR Green I dye, and MgCl2 at a final concentration of 2.5 mM.Where noted, Master Mixes contain instrument-specific reference dyes, while the Universal kit includes 50X ROX High and 50X ROX Low separately.

Preparation Note

Always ensure that components have been fully thawed and thoroughly mixed before use. The KAPA SYBR FAST qPCR Master Mix (2X) may not freeze solidly, even when stored at -20°C. The KAPA RT Mix is temperaturesensitive, and should be stored at -20°C and kept on ice during use.

The SYBR Green I dye contained in the KAPA SYBR FAST qPCR Master Mix (2X) and ROX/fluorescein dyes (depending on kit configuration) are light sensitive. Exposure to direct light for an extended period of time will result in loss of fluorescent signal intensity.

KAPA SYBR FAST qPCR Master Mix (2X) is stable through 30  freeze-thaw cycles. Ensure that all reagents are stored protected from light at -20°C when not in use. When protected from light, reagents are stable in the dark at 4°C for at least one week and may be stored at this temperature for short-term use provided that they do not become contaminated with microbes and/or nucleases.

Quality

All kit components are subjected to stringent functional quality control, are free of detectable contaminating exo and endonuclease activity, and meet strict requirements with respect to DNA contamination.

Application

• Gene expression analysis
• Low-copy gene detection
• Microarray validationmiRNA research
• Gene knockdown validation

Features and Benefits

Increase signal and reaction efficiency
• Consistently higher amplification efficiencies for accurate qPCR.
• Higher fluorescence, earlier Cq values and improved reaction efficiencies.

Amplify across a broad range of target lengths
• Robust performance independent of amplicon size.
• Improve sensitivity and reproducibility
• Accurate interrogation across a wide range of RNA template concentrations.

Quick Notes:
• This kit contains wild-type M-MuLV and an engineered enzyme optimized for qPCR using SYBR Green I dye chemistry.
• The 2X master mix contains a proprietary buffer. Together with the novel enzyme, this improves amplification efficiency of both GC- and AT-rich targets.
• Use only gene-specific primers for one-step qRT-PCR.
• Optimal cDNA synthesis is achieved at 42°C for 5 min.
• 3 min at 95°C is sufficient for RT inactivation and DNA polymerase activation.
• For 3-step cycling, use 20 sec for primer annealing and 1 sec for extension/data acquisition at 72°C.
• Do not exceed 25 μL reaction volumes.

General description

KAPA SYBR FAST One-Step qRT-PCR Kits are designed for optimal performance in stringent qRT-PCR reaction conditions, exhibiting dramatic improvements in sensitivity, specificity, reaction efficiency, and fluorescence. The kit is optimized for rapid one-step, one-tube RNA quantification, reducing experimental variation and contamination with a convenient qRT-PCR protocol.

KAPA SYBR FAST One-Step qRT-PCR Kits contain M-MuLV reverse transcriptase, RNase inhibitor, and a novel DNA polymerase evolved via directed evolution.

Legal Information

SYBR is a registered trademark of Life Technologies

Other Notes

For Research Use Only. Not for use in diagnostic procedures.

Price and Availability

Safety & Documentation

Safety Information

Symbol 
GHS08  GHS08
Signal word 
Warning
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

Protocols & Articles

Articles

KAPA SYBR® FAST One-Step qRT-PCR Kits FAQs

What is the difference between a one-step and a two-step qRT-PCR reaction? In a one-step qRT-PCR reaction, reverse transcription and quantitative PCR are performed as a single, closed tube assay, red...
Keywords: Amplification, Degradations, Gene expression, Gene knockdown, High performance liquid chromatography, Microarray Analysis, Nucleic acid annealing, Polymerase chain reaction, Polymerase chain reaction - quantitative, Transcription

Related Content

PCR Selection Guide

Sigma-Aldrich offers a wide variety of PCR reagents to meet any experimental needs. Our range of polymerases is customized to meet your End-Point PCR, qPCR, or RT-PCR needs. Our products vary from ro...
Keywords: Polymerase chain reaction, Polymerase chain reaction - quantitative

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?